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Drug-repositioning screening identified piperlongumine as a direct STAT3 inhibitor with potent activity against breast cancer.
- Source :
-
Oncogene [Oncogene] 2015 Mar 12; Vol. 34 (11), pp. 1341-53. Date of Electronic Publication: 2014 Mar 31. - Publication Year :
- 2015
-
Abstract
- Signal transducer and activator of transcription (STAT) 3 regulates many cardinal features of cancer including cancer cell growth, apoptosis resistance, DNA damage response, metastasis, immune escape, tumor angiogenesis, the Warburg effect and oncogene addiction and has been validated as a drug target for cancer therapy. Several strategies have been used to identify agents that target Stat3 in breast cancer but none has yet entered into clinical use. We used a high-throughput fluorescence microscopy search strategy to identify compounds in a drug-repositioning library (Prestwick library) that block ligand-induced nuclear translocation of Stat3 and identified piperlongumine (PL), a natural product isolated from the fruit of the pepper Piper longum. PL inhibited Stat3 nuclear translocation, inhibited ligand-induced and constitutive Stat3 phosphorylation, and modulated expression of multiple Stat3-regulated genes. Surface plasmon resonance assay revealed that PL directly inhibited binding of Stat3 to its phosphotyrosyl peptide ligand. Phosphoprotein antibody array analysis revealed that PL does not modulate kinases known to activate Stat3 such as Janus kinases, Src kinase family members or receptor tyrosine kinases. PL inhibited anchorage-independent and anchorage-dependent growth of multiple breast cancer cell lines having increased pStat3 or total Stat3, and induced apoptosis. PL also inhibited mammosphere formation by tumor cells from patient-derived xenografts. PL's antitumorigenic function was causally linked to its Stat3-inhibitory effect. PL was non-toxic in mice up to a dose of 30 mg/kg/day for 14 days and caused regression of breast cancer cell line xenografts in nude mice. Thus, PL represents a promising new agent for rapid entry into the clinic for use in treating breast cancer, as well as other cancers in which Stat3 has a role.
- Subjects :
- Active Transport, Cell Nucleus drug effects
Animals
Antineoplastic Agents adverse effects
Antineoplastic Agents therapeutic use
Apoptosis drug effects
Cell Proliferation drug effects
Dioxolanes adverse effects
Dioxolanes therapeutic use
Female
Gene Expression drug effects
Humans
MCF-7 Cells
Mice
Mice, Nude
Mice, SCID
Neoplasm Transplantation
Oxidative Stress drug effects
Phosphorylation drug effects
Reverse Transcriptase Polymerase Chain Reaction
STAT3 Transcription Factor genetics
STAT3 Transcription Factor metabolism
Signal Transduction drug effects
Spheroids, Cellular drug effects
Surface Plasmon Resonance
Transplantation, Heterologous
Tumor Cells, Cultured
Antineoplastic Agents pharmacology
Breast Neoplasms drug therapy
Dioxolanes pharmacology
Drug Repositioning
STAT3 Transcription Factor antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 1476-5594
- Volume :
- 34
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Oncogene
- Publication Type :
- Academic Journal
- Accession number :
- 24681959
- Full Text :
- https://doi.org/10.1038/onc.2014.72