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Ethanol production from glycerol-containing biodiesel waste by Klebsiella variicola shows maximum productivity under alkaline conditions.
- Source :
-
New biotechnology [N Biotechnol] 2014 May 25; Vol. 31 (3), pp. 246-53. Date of Electronic Publication: 2014 Mar 26. - Publication Year :
- 2014
-
Abstract
- Biodiesel fuel (BDF) waste contains large amounts of crude glycerol as a by-product, and has a high alkaline pH. With regard to microbial conversion of ethanol from BDF-derived glycerol, bacteria that can produce ethanol at alkaline pH have not been reported to date. Isolation of bacteria that shows maximum productivity under alkaline conditions is essential to effective production of ethanol from BDF-derived glycerol. In this study, we isolated the Klebsiella variicola TB-83 strain, which demonstrated maximum ethanol productivity at alkaline pH. Strain TB-83 showed effective usage of crude glycerol with maximum ethanol production at pH 8.0-9.0, and the culture pH was finally neutralized by formate, a by-product. In addition, the ethanol productivity of strain TB-83 under various culture conditions was investigated. Ethanol production was more efficient with the addition of yeast extract. Strain TB-83 produced 9.8 g/L ethanol (0.86 mol/mol glycerol) from cooking oil-derived BDF waste. Ethanol production from cooking oil-derived BDF waste was higher than that of new frying oil-derived BDF and pure-glycerol. This is the first report to demonstrate that the K. variicola strain TB-83 has the ability to produce ethanol from glycerol at alkaline pH.<br /> (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Subjects :
- Base Sequence
Batch Cell Culture Techniques
Hydrogen-Ion Concentration
Klebsiella genetics
Klebsiella growth & development
Klebsiella isolation & purification
Metabolome drug effects
Phylogeny
Potassium Chloride pharmacology
RNA, Ribosomal, 16S genetics
Salts pharmacology
Sodium Chloride pharmacology
Temperature
Alkalies pharmacology
Biofuels microbiology
Biotechnology methods
Ethanol metabolism
Glycerol metabolism
Klebsiella metabolism
Waste Products
Subjects
Details
- Language :
- English
- ISSN :
- 1876-4347
- Volume :
- 31
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- New biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 24681408
- Full Text :
- https://doi.org/10.1016/j.nbt.2014.03.005