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Functional and molecular characterization of a lipopeptide surfactant from the marine sponge-associated eubacteria Bacillus licheniformis NIOT-AMKV06 of Andaman and Nicobar Islands, India.
- Source :
-
Marine pollution bulletin [Mar Pollut Bull] 2014 May 15; Vol. 82 (1-2), pp. 76-85. Date of Electronic Publication: 2014 Mar 27. - Publication Year :
- 2014
-
Abstract
- The production of a lipopeptide surfactant from the sponge-associated eubacteria Bacillus licheniformis NIOT-AMKV06 from the Andaman and Nicobar Islands was investigated. The highest production was attained with glucose and yeast extracts as the carbon and nitrogen sources (1.789 mg mL(-1)), respectively. The surfactant was highly stable over a pH range of 5.0-10 and a temperature range of 20-70°C with high NaCl concentrations. Excellent emulsification activity was exhibited by the purified surfactant with crude oil, kerosene, and diesel. A two-fold increase in surfactant production (3.0 mg mL(-1)) was observed using the newly formulated medium in this study. The surfactant biosynthesis gene cluster (sfp, sfpO, and srfA) from B. licheniformis NIOT-AMKV06 was heterologously expressed in Escherichia coli, and the production was increased three-fold (11.78 g L(-1)) over the original strain. The results confirm the potential of the surfactant for use in bioremediation of hydrocarbons in a marine environment and for enhanced oil recovery. To our knowledge, this is the first report on the ability of a hydrocarbon degrading B. licheniformis from marine sponges for the biosynthesis of a potent lipopeptide surfactant possessing characteristics of maximum stability, outstanding surfactant activity, and exceptional emulsifying capability.<br /> (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Bacillus metabolism
DNA, Bacterial genetics
DNA, Bacterial metabolism
India
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S genetics
RNA, Ribosomal, 16S metabolism
Bacillus chemistry
Bacillus genetics
Bacterial Proteins genetics
Bacterial Proteins metabolism
Lipopeptides genetics
Lipopeptides metabolism
Surface-Active Agents metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3363
- Volume :
- 82
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Marine pollution bulletin
- Publication Type :
- Academic Journal
- Accession number :
- 24680716
- Full Text :
- https://doi.org/10.1016/j.marpolbul.2014.03.018