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Mutagenic treatments result in inactivation of expression of a transfected bacterial gene integrated into a human cell line.
- Source :
-
Mutation research [Mutat Res] 1989 Mar-May; Vol. 220 (2-3), pp. 255-62. - Publication Year :
- 1989
-
Abstract
- The cell line E2 is a SV40-transformed human fibroblast cell line containing a single integrated copy of the bacterial guanine phosphoribosyl transferase (gpt) gene. Treatment of E2 with ultraviolet light (UV) or ethyl methanesulphonate (EMS) induced the formation of Gpt- derivatives. Several induced derivatives have been isolated, and the structure, expression and revertibility of the gpt gene have been analysed. In the majority of cases the Gpt- phenotype resulted from switching off the gpt gene, in most instances by methylation, but in a few cases by phenotypic switching. Thus mutagenic treatment can result in the inactivation of gene expression in human cells. In a small proportion of Gpt- derivatives the gpt sequences were deleted.
Details
- Language :
- English
- ISSN :
- 0027-5107
- Volume :
- 220
- Issue :
- 2-3
- Database :
- MEDLINE
- Journal :
- Mutation research
- Publication Type :
- Academic Journal
- Accession number :
- 2467202
- Full Text :
- https://doi.org/10.1016/0165-1110(89)90029-8