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Histologic examinations of teeth treated with 2 scaffolds: a pilot animal investigation.
- Source :
-
Journal of endodontics [J Endod] 2014 Apr; Vol. 40 (4), pp. 515-20. Date of Electronic Publication: 2014 Feb 12. - Publication Year :
- 2014
-
Abstract
- Introduction: A growing body of evidence is building a case for the possibility of tissue regeneration within the root canal of necrotic teeth, allowing for continued root development. However, it remains unknown what type of tissue is produced after regenerative endodontics. The purpose of this study was to use blood clots and platelet-rich plasma (PRP) as scaffolds in regenerative endodontics under ideal conditions in a ferret model to examine the tissues generated within the root canals.<br />Methods: The pulps of 21 canine teeth from 7 young ferrets were extirpated using broaches without filing the canal walls. Bleeding was stimulated from the periapical tissues, and a blood clot was induced in the canal space to the level of the cementoenamel junction in 12 teeth. PRP was prepared and placed in the canals to the level of the cementoenamel junction in 9 teeth. The coronal access was sealed with mineral trioxide aggregate. Seven canines were not operated on and served as controls. Three months later, block sections including each canine and its surrounding tissues were removed for histologic evaluation. The tissues found in the canals of experimental teeth were compared with those in the control teeth.<br />Results: Almost all of the experimental teeth showed the presence of intracanal bonelike tissue. No evidence of dentinal wall thickening or apical narrowing was noted in the experimental teeth.<br />Conclusions: In this experimental model, the use of either PRP or blood clots during regenerative endodontics leads to the formation of intracanal bonelike tissue without continual root maturation.<br /> (Copyright © 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Aluminum Compounds therapeutic use
Animals
Calcium Compounds therapeutic use
Cuspid physiology
Dental Pulp Cavity blood supply
Dental Pulp Cavity physiology
Dentin, Secondary anatomy & histology
Dentin, Secondary physiology
Drug Combinations
Edetic Acid therapeutic use
Ferrets
Fibroblasts physiology
Lymphocytes physiology
Macrophages physiology
Male
Models, Animal
Odontogenesis physiology
Osteoblasts physiology
Osteoclasts physiology
Oxides therapeutic use
Pulpectomy methods
Random Allocation
Regeneration physiology
Root Canal Filling Materials therapeutic use
Root Canal Irrigants therapeutic use
Silicates therapeutic use
Time Factors
Blood Coagulation physiology
Cuspid anatomy & histology
Dental Pulp Cavity anatomy & histology
Platelet-Rich Plasma physiology
Tissue Scaffolds
Subjects
Details
- Language :
- English
- ISSN :
- 1878-3554
- Volume :
- 40
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of endodontics
- Publication Type :
- Academic Journal
- Accession number :
- 24666902
- Full Text :
- https://doi.org/10.1016/j.joen.2013.12.025