Back to Search
Start Over
Puerarin alleviates aggravated sympathoexcitatory response induced by myocardial ischemia via regulating P2X3 receptor in rat superior cervical ganglia.
- Source :
-
Neurochemistry international [Neurochem Int] 2014 May; Vol. 70, pp. 39-49. Date of Electronic Publication: 2014 Mar 18. - Publication Year :
- 2014
-
Abstract
- Myocardial ischemia elicits a sympathoexcitatory response characterized by increase in blood pressure and sympathetic nerve activity. Puerarin, a major active ingredient extracted from the traditional Chinese plant medicine Ge-gen, has been widely used in treatment of myocardial and cerebral ischemia. However, little is known about the mechanism. Our study was aimed to explore the effect of puerarin on sympathoexcitatory response induced by myocardial ischemic injury and possible relationship with P2X3 receptor. Our results showed that puerarin alleviated systolic blood pressure and heart rate, and decreased the up-regulated of P2X3 mRNA and protein in SCG of myocardial ischemic rats. The amplitude of ATP-activated currents of SCG neurons was much larger in myocardial ischemic group than that in control group. Puerarin reduced ATP-activated currents in myocardial ischemic group and control group, and the inhibiting effects of puerarin in myocardial ischemic group were stronger than those in control group. Puerarin also significantly inhibited ATP-activated currents in HEK293 cells transfected with P2X3 receptor. These results suggest that puerarin can depress up-sympathoexcitatory response induced by myocardial ischemia via acting on P2X3 receptor in rat SCG to protect myocardium.<br /> (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Blood Pressure drug effects
Cells, Cultured
Humans
Myocardial Ischemia metabolism
Neurons metabolism
Rats, Sprague-Dawley
Superior Cervical Ganglion metabolism
Sympathetic Nervous System metabolism
Isoflavones pharmacology
Myocardial Ischemia drug therapy
Receptors, Purinergic P2X3 metabolism
Signal Transduction drug effects
Superior Cervical Ganglion drug effects
Vasodilator Agents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1872-9754
- Volume :
- 70
- Database :
- MEDLINE
- Journal :
- Neurochemistry international
- Publication Type :
- Academic Journal
- Accession number :
- 24657446
- Full Text :
- https://doi.org/10.1016/j.neuint.2014.03.004