Back to Search
Start Over
Cholera toxin B subunit-binding and ganglioside GM1 immuno-expression are not necessarily correlated in human salivary glands.
- Source :
-
Acta odontologica Scandinavica [Acta Odontol Scand] 2014 Nov; Vol. 72 (8), pp. 694-700. Date of Electronic Publication: 2014 Mar 21. - Publication Year :
- 2014
-
Abstract
- Objective: To determine and compare the presence and in situ localization of the glycosphingolipid ganglioside GM1 in human salivary glands using the biomarkers for GM1: cholera toxin and antibodies against GM1.<br />Materials and Methods: Immunohistochemical analyses were performed on sections of adult human submandibular, parotid and palatinal glands using cholera toxin sub-unit B and two polyclonal antibodies against ganglioside GM1 as biomarkers.<br />Results: Immunofluorescence microscopy showed that the toxin and antibodies were co-localized in some acini but not in others. The cholera toxin mainly reacted with the cell membranes of the mucous acini in the submandibular gland, while incubation with the antibody against GM1 gave rise to a staining of the cytoplasm. The cytoplasm in some secretory acinar cells in the parotid gland was stained by the cholera toxin, whereas only small spots on the plasma membranes reacted with anti-GM1. The plasma membranes in the parotid excretory ducts appeared to react to anti-GM1, but not to cholera toxin.<br />Conclusions: Cholera toxin induces the expression of ion channels and carriers in the small intestine and increases the production of secretory mucins. Although their mutual immunohistochemical localization may differ, both cholera toxin and ganglioside GM1 are present in the mucin-producing acini from salivary glands. This could point to a relationship between ganglioside expression and production of salivary mucins.
- Subjects :
- Adult
Antibodies
Biomarkers
Cadaver
Cell Membrane chemistry
Cell Membrane ultrastructure
Cytoplasm chemistry
Cytoplasm ultrastructure
Humans
Immunohistochemistry
Microscopy, Fluorescence
Mucins chemistry
Parotid Gland chemistry
Parotid Gland cytology
Salivary Ducts chemistry
Salivary Ducts cytology
Salivary Glands cytology
Salivary Glands, Minor chemistry
Salivary Glands, Minor cytology
Serous Membrane chemistry
Serous Membrane cytology
Submandibular Gland chemistry
Submandibular Gland cytology
Cholera Toxin
G(M1) Ganglioside analysis
Salivary Glands chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1502-3850
- Volume :
- 72
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Acta odontologica Scandinavica
- Publication Type :
- Academic Journal
- Accession number :
- 24655314
- Full Text :
- https://doi.org/10.3109/00016357.2014.898090