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Constitutive production of procollagenase and tissue inhibitor of metalloproteinases by human keratinocytes in culture.
- Source :
-
The Journal of investigative dermatology [J Invest Dermatol] 1989 Feb; Vol. 92 (2), pp. 156-9. - Publication Year :
- 1989
-
Abstract
- Production of procollagenase and tissue inhibitor of metalloproteinases was demonstrated in human keratinocyte cultures. The two proteins were immunoprecipitated from keratinocyte-conditioned medium with antibodies to human dermal fibroblast collagenase and tissue inhibitor of metalloproteinases and quantitated with enzyme-linked immunosorbent assays. Treatment of the keratinocytes with the phorbol ester, 12-0-tetradecanoylphorbol-13-acetate, produced a six to 34-fold increase in procollagenase synthesis and secretion but only a threefold increase in the production of tissue inhibitor of metalloproteinases. Collagenase and tissue inhibitor of metalloproteinases mRNAs were present in normal keratinocytes, were the same size as their fibroblast counterparts, and both increased in response to treatment with 12-0-tetradecanoylphorbol-13-acetate. These data suggest that remodeling of type I collagen may be an important function of human keratinocytes in vivo.
- Subjects :
- Cells, Cultured
Enzyme Inhibitors genetics
Enzyme Precursors genetics
Epidermis enzymology
Epidermis metabolism
Humans
Metalloendopeptidases antagonists & inhibitors
Microbial Collagenase genetics
Precipitin Tests
RNA, Messenger metabolism
Tetradecanoylphorbol Acetate pharmacology
Tissue Inhibitor of Metalloproteinases
Collagenases
Enzyme Inhibitors biosynthesis
Enzyme Precursors biosynthesis
Epidermal Cells
Keratins
Microbial Collagenase biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0022-202X
- Volume :
- 92
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of investigative dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 2465348
- Full Text :
- https://doi.org/10.1111/1523-1747.ep12276678