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Islet amyloid polypeptide is not a target antigen for CD8+ T-cells in type 2 diabetes.

Authors :
Kalantar F
Dabbaghmanesh MH
Martinuzzi E
Moghadami M
Amirghofran Z
Source :
Iranian journal of immunology : IJI [Iran J Immunol] 2014 Mar; Vol. 11 (1), pp. 1-12.
Publication Year :
2014

Abstract

Background: Type 2 diabetes (T2D) is a chronic metabolic disorder in which beta-cells are destroyed. The islet amyloid polypeptide (IAPP) produced by beta-cells has been reported to influence beta-cell destruction.<br />Objective: To evaluate if IAPP can act as an autoantigen and therefore, to see if CD8+ T-cells specific for this protein might be present in T2D patients.<br />Methods: Peripheral blood mononuclear cells (PBMC) were obtained from human leukocyte antigen (HLA)-A2+ T2D patients and non-diabetic healthy subjects. Cells were then screened for peptide recognition using ELISPOT assay for the presence of IFN-γ producing CD8+ T-cells against two HLA Class I-restricted epitopes derived from IAPP (IAPP5-13 and IAPP9-17) and common viral antigenic minimal epitopes Flu MP 58-66, CMV495-503, EBV280-288 and HIV77-85 as controls.<br />Results: A total of 36.4% of patients and 56.2% of healthy subjects showed a response against IAPP5-13 peptide. No significant difference in response against this peptide was noted between the patients and the healthy donors. With respect to peptide IAPP9-17, although healthy subjects showed a higher mean number of spot forming cells than the patients, the difference was not significant; 36.4% of patients and 37.5% of controls responded to this peptide. The response of healthy subjects to the common viral peptides was stronger than that of the patients, though the result was not significant.<br />Conclusions: It is unlikely that IAPP would be a target for CD8+ T-cells in diabetic patients; however, the trend observed toward a lower response of T2D patients against IAPP and common viral peptides may imply a decreased immune response in these patients.

Details

Language :
English
ISSN :
1735-367X
Volume :
11
Issue :
1
Database :
MEDLINE
Journal :
Iranian journal of immunology : IJI
Publication Type :
Academic Journal
Accession number :
24632583
Full Text :
https://doi.org/IJIv11i1A1