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Role of vitamin D derivatives in intestinal tissue of patients with inflammatory bowel diseases.
- Source :
-
Journal of Crohn's & colitis [J Crohns Colitis] 2014 Sep; Vol. 8 (9), pp. 1062-71. Date of Electronic Publication: 2014 Mar 12. - Publication Year :
- 2014
-
Abstract
- Background and Aim: The adhesion molecule expression and matrix metalloproteinases (MMPs) are proposed to be major factors for intestinal injury mediated by T cells in (IBD) and are up-regulated in intestinal mucosa of IBD patients. To investigate the effect of vitamin D derivatives on adhesion molecules and MMPs in colonic biopsies of IBD patients.<br />Methods: Biopsies from inflamed and non-inflamed tract of terminal ileum and colon and PBMC from the same IBD patients were cultured with or without vitamin D derivatives. MMP activity and adhesion molecule levels were determined.<br />Results: 1,25(OH)2D3 and ZK 191784 significantly decrease ICAM-1 protein levels in the biopsies obtained only from the inflamed region of intestine of UC patients, while MAdCAM-1 levels decrease in the presence of 1,25(OH)2D3 in the non-inflamed region, and, in the presence of ZK, in the inflamed one. In CD patients 1,25(OH)2D3 and ZK decrease ICAM-1 and MAdCAM-1 in the biopsies obtained from the non-inflamed and inflamed regions, with the exception of ICAM-1 in the inflamed region in the presence of 1,25(OH)2D3. The expression of MMP-9, MMP-2, and MMP-3 decreases in the presence of vitamin D derivatives in UC and CD with the exception of 1,25(OH)2D3 that does not affect the levels of MMP-9 and MMP-2 in CD. Vitamin D derivatives always affect MMP-9, MMP-2 and ICAM-1 in PBMC of UC and CD patients.<br />Conclusions: Based on the increased expression of ICAM-1, MAdCAM-1 and MMP-2,-9,-3 in IBD, our study suggests that vitamin D derivatives may be effective in the management of these diseases.<br /> (Copyright © 2014 European Crohn's and Colitis Organisation. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Biopsy
Blotting, Western
Calcitriol therapeutic use
Cell Adhesion Molecules
Cells, Cultured
Humans
Immunoglobulins drug effects
Inflammatory Bowel Diseases drug therapy
Inflammatory Bowel Diseases pathology
Intercellular Adhesion Molecule-1 drug effects
Intestinal Mucosa drug effects
Intestinal Mucosa metabolism
Matrix Metalloproteinases drug effects
Mucoproteins drug effects
Vitamin D analogs & derivatives
Vitamins
Calcitriol analogs & derivatives
Hydroxycholecalciferols therapeutic use
Immunoglobulins metabolism
Inflammatory Bowel Diseases metabolism
Intercellular Adhesion Molecule-1 metabolism
Intestinal Mucosa pathology
Matrix Metalloproteinases metabolism
Mucoproteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1876-4479
- Volume :
- 8
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Journal of Crohn's & colitis
- Publication Type :
- Academic Journal
- Accession number :
- 24630484
- Full Text :
- https://doi.org/10.1016/j.crohns.2014.02.005