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Extravascular optical coherence tomography: evaluation of carotid atherosclerosis and pravastatin therapy.
- Source :
-
Stroke [Stroke] 2014 Apr; Vol. 45 (4), pp. 1123-1130. Date of Electronic Publication: 2014 Mar 13. - Publication Year :
- 2014
-
Abstract
- Background and Purpose: Extravascular optical coherence tomography (OCT), as a noninvasive imaging methodology with micrometer resolution, was evaluated in a murine model of carotid atherosclerosis by way of assessing the efficacy of pravastatin therapy.<br />Methods: An OCT device was engineered for extravascular plaque imaging. Wild-type mice and apolipoprotein E-deficient (ApoE(-/-)) mice were randomized to 3 treatment groups: (1) wild-type on a diet of standard rodent chow (n=13); (2) ApoE(-/-) on a high-fat, atherosclerotic diet (HFD; n=13); and (3) ApoE(-/-) on a HFD given daily pravastatin (n=13). Mice were anesthetized and the left common carotid was surgically exposed. Three-dimensional (3D; 2 spatial dimensions+time) and 4D (3 spatial dimensions+time) OCT images of the vessel lumen patency were evaluated. After perfusion, in situ OCT imaging was performed for statistical comparison with the in vivo results and final histology.<br />Results: Intraoperative OCT imaging positively identified carotid plaque in 100% of ApoE(-/-) mice on HFD. ApoE(-/-) mice on HFD had a significantly decreased lumen patency when compared with that in wild-type mice (P<0.001). Pravastatin therapy was found to increase lumen patency significantly in ApoE(-/-) mice on HFD (P<0.01; compared with ApoE(-/-) on HFD). The findings were confirmed with OCT imaging after perfusion and histology.<br />Conclusions: OCT imaging offers the potential for real-time, detailed vessel lumen evaluation, potentially improving surgical accuracy and outcomes during cerebrovascular neurosurgical procedures. Pravastatin significantly increases vessel lumen patency in the ApoE(-/-) mouse on HFD.
- Subjects :
- Animals
Apolipoproteins E genetics
Carotid Stenosis drug therapy
Carotid Stenosis pathology
Disease Models, Animal
Hydroxymethylglutaryl-CoA Reductase Inhibitors pharmacology
Imaging, Three-Dimensional methods
Mice
Mice, Inbred C57BL
Mice, Knockout
Random Allocation
Carotid Artery Diseases drug therapy
Carotid Artery Diseases pathology
Drug Monitoring methods
Pravastatin pharmacology
Tomography, Optical Coherence methods
Subjects
Details
- Language :
- English
- ISSN :
- 1524-4628
- Volume :
- 45
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Stroke
- Publication Type :
- Academic Journal
- Accession number :
- 24627118
- Full Text :
- https://doi.org/10.1161/STROKEAHA.113.002970