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Ionic currents in avian granulosa cells.
- Source :
-
FEBS letters [FEBS Lett] 1988 Dec 05; Vol. 241 (1-2), pp. 169-72. - Publication Year :
- 1988
-
Abstract
- Transmembrane ionic currents have been recorded in single granulosa cells from the laying hen using the whole-cell patch-clamp technique. Under voltage-clamp conditions, depolarizing voltage steps evoked currents composed of a fast inactivating inward component and a delayed outward component. The former was activated at voltages more positive than -50 mV and was fully inactivated within 500 ms. It was blocked by D600 (methoxyverapamil) and by cobalt, suggesting that it is a calcium current. The latter displayed inward rectification and did not inactivate during long duration pulses. It was blocked by tetraethylammonium indicating that it is a potassium current. This is the first evidence of the existence of potassium and calcium transmembrane currents in granulosa cells.
- Subjects :
- Animals
Barium pharmacology
Calcium pharmacology
Calcium Channels drug effects
Calcium Channels physiology
Cells, Cultured
Chickens
Female
Gallopamil pharmacology
Membrane Potentials drug effects
Potassium Channels drug effects
Potassium Channels physiology
Tetraethylammonium
Tetraethylammonium Compounds pharmacology
Granulosa Cells physiology
Ion Channels physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 241
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 2461875
- Full Text :
- https://doi.org/10.1016/0014-5793(88)81053-6