Glucuronidation of aurantio-obtusin: identification of human UDP-glucuronosyltransferases and species differences.

Authors :: Mi BL
Sun Q
Qu YQ
Gao XX
Yu ZW
Ge GB
Cai SS
Zhang J
Zheng YC
Zhang ZQ
Source :: Xenobiotica; the fate of foreign compounds in biological systems [Xenobiotica] 2014 Aug; Vol. 44 (8), pp. 716-21. Date of Electronic Publication: 2014 Mar 11.
Publication Year :: 2014
Abstract: 1. The aurantio-obtusin's glucuronide was detected when aurantio-obtusin was incubated with human liver microsomes (HLMs). Recombinant UGT isoforms screening experiment showed that UGT1A8 was the major isoform contributed to the glucuronidation. 2. The metabolic profiles for aurantio-obtusin in liver microsomes from different species were similar, however, the intrinsic clearance values (Vmax/Km) among the species were: Monkey > Human > Rat > Rabbit > Dog > Pig > Mouse > Guinea pig.

Subjects :: Animals
Enzyme Assays
Glucuronides chemistry
Humans
Kinetics
Mass Spectrometry
Microsomes, Liver metabolism
Recombinant Proteins metabolism
Species Specificity
Anthraquinones metabolism
Glucuronides metabolism
Glucuronosyltransferase metabolism

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