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Antimicrobial susceptibility of Salmonella isolates from healthy pigs and chickens (2008-2011).
- Source :
-
Veterinary microbiology [Vet Microbiol] 2014 Jul 16; Vol. 171 (3-4), pp. 298-306. Date of Electronic Publication: 2014 Feb 07. - Publication Year :
- 2014
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Abstract
- Using the agar dilution method, antimicrobial susceptibility to human-use antibiotics was determined among Belgian faecal Salmonella isolates from healthy pigs and broiler chickens. Both epidemiological cut-off values and clinical breakpoints were applied for interpretation of the results. Cephalosporin-resistant isolates were examined for the presence of genes encoding CTX-M, SHV, TEM and CMY β-lactamases. All isolates with decreased quinolone susceptibility were screened for plasmid-borne genes qnr, qepA and aac(6')-Ib-cr. In all, 368 Salmonella isolates were recovered from pigs and 452 from chickens. Clinical resistance to ciprofloxacin was absent in isolates of both host species, and was 1.9 and 13.1% to cefotaxime in pig and poultry isolates, respectively. Decreased susceptibility to cefotaxime amounted to 2.2 and 0.7%, whereas for ciprofloxacin this was 3.0 and 23.0% in pig and poultry isolates, respectively. Ciprofloxacin decreased susceptibility was limited to few serovars, mainly Paratyphi B. Multidrug resistance was markedly higher for pig isolates (39.7%) than for chicken isolates (17.3%). Sixty-six cefotaxime-resistant isolates, 59 from chickens and 7 from pigs, were phenotypically determined as ESBL/AmpC producers; predominantly Paratyphi B and Typhimurium serovars. BlaCTX-M (mostly blaCTXM-1, but also blaCTXM-2 and blaCTXM-9) and blaTEM-52 were the predominant ESBL genes. Only few isolates expressed SHV-12 or an AmpC enzyme (CMY-2). Isolates of four serovars carried qnr genes: Brandenburg and Llandof from pigs, both qnrS; Indiana and Paratyphi B from chickens with qnrB and qnrA. The latter isolate carried blaCTX-M-9 and was the only strain with a plasmid-borne quinolone resistance gene among the ESBL/AmpC producers. This Salmonella survey confirms that the ESBL/AmpC producers are particularly prevalent in chickens (12.8%), and much less in pigs (1.9%). A link between plasmid-borne quinolone resistance genes and ESBLs/AmpC was uncommon.<br /> (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Bacterial Proteins genetics
Belgium
Chickens genetics
Feces microbiology
Humans
Microbial Sensitivity Tests statistics & numerical data
Microbial Sensitivity Tests veterinary
Salmonella genetics
Salmonella isolation & purification
Species Specificity
Sus scrofa genetics
Swine
beta-Lactamases genetics
Anti-Infective Agents pharmacology
Cephalosporins pharmacology
Chickens microbiology
Ciprofloxacin pharmacology
Drug Resistance, Multiple, Bacterial genetics
Salmonella drug effects
Sus scrofa microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2542
- Volume :
- 171
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Veterinary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 24598135
- Full Text :
- https://doi.org/10.1016/j.vetmic.2014.01.030