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Citrus tristeza virus-based RNAi in citrus plants induces gene silencing in Diaphorina citri, a phloem-sap sucking insect vector of citrus greening disease (Huanglongbing).
- Source :
-
Journal of biotechnology [J Biotechnol] 2014 Apr 20; Vol. 176, pp. 42-9. Date of Electronic Publication: 2014 Feb 23. - Publication Year :
- 2014
-
Abstract
- A transient expression vector based on Citrus tristeza virus (CTV) is unusually stable. Because of its stability it is being considered for use in the field to control Huanglongbing (HLB), which is caused by Candidatus Liberibacter asiaticus (CLas) and vectored by Asian citrus psyllid, Diaphorina citri. In the absence of effective control strategies for CLas, emphasis has been on control of D. citri. Coincident cohabitation in phloem tissue by CLas, D. citri and CTV was exploited to develop a novel method to mitigate HLB through RNA interference (RNAi). Since CTV has three RNA silencing suppressors, it was not known if CTV-based vector could induce RNAi in citrus. Yet, expression of sequences targeting citrus phytoene desaturase gene by CTV-RNAi resulted in photo-bleaching phenotype. CTV-RNAi vector, engineered with truncated abnormal wing disc (Awd) gene of D. citri, induced altered Awd expression when silencing triggers ingested by feeding D. citri nymphs. Decreased Awd in nymphs resulted in malformed-wing phenotype in adults and increased adult mortality. This impaired ability of D. citri to fly would potentially limit the successful vectoring of CLas bacteria between citrus trees in the grove. CTV-RNAi vector would be relevant for fast-track screening of candidate sequences for RNAi-mediated pest control.<br /> (Copyright © 2014. Published by Elsevier B.V.)
- Subjects :
- Animals
Citrus genetics
Closterovirus classification
Gene Silencing
Hemiptera physiology
Nymph genetics
Oxidoreductases genetics
Phloem microbiology
Plant Diseases microbiology
Plant Proteins genetics
Citrus microbiology
Closterovirus genetics
Genes, Insect
Hemiptera genetics
RNA Interference
RNA, Viral genetics
Rhizobiaceae physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4863
- Volume :
- 176
- Database :
- MEDLINE
- Journal :
- Journal of biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 24572372
- Full Text :
- https://doi.org/10.1016/j.jbiotec.2014.02.010