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A widely applicable plasma renin activity assay by LC-MS/MS with offline solid phase extraction.
- Source :
-
Annals of clinical biochemistry [Ann Clin Biochem] 2014 May; Vol. 51 (Pt 3), pp. 409-11. Date of Electronic Publication: 2014 Feb 25. - Publication Year :
- 2014
-
Abstract
- Background: The measurement of plasma renin activity using LC-MS/MS is an attractive alternative to radioimmunoassay techniques. A published method for renin activity by LC-MS/MS uses equipment that is not available in all LC-MS/MS laboratories. Here, we present an offline modification to allow users of LC-MS/MS to perform this analysis without specialist equipment.<br />Methods: Samples were prepared in duplicate and incubated for 6.5 h and 24 h. Solid phase extraction was performed offline using Waters Oasis MAX ion-exchange 96-well plate. A method comparison was performed between this assay and a previously published assay using on-line solid phase extraction.<br />Results: The offline method for plasma rennin activity gave similar results to the on-line method across the concentration range. Analytical performance was also found to be comparable and the use of a 24 h incubation for low samples was also deemed unnecessary.<br />Discussion: The method described is an acceptable alternative to a previously published assay which does not require the use of highly specialist equipment and will be applicable to the majority of LC-MS/MS users.
Details
- Language :
- English
- ISSN :
- 1758-1001
- Volume :
- 51
- Issue :
- Pt 3
- Database :
- MEDLINE
- Journal :
- Annals of clinical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 24569391
- Full Text :
- https://doi.org/10.1177/0004563214524558