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Correlation between presence of Trypanosoma cruzi DNA in heart tissue of baboons and cynomolgus monkeys, and lymphocytic myocarditis.
- Source :
-
The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 2014 Apr; Vol. 90 (4), pp. 627-33. Date of Electronic Publication: 2014 Feb 24. - Publication Year :
- 2014
-
Abstract
- Trypanosoma cruzi, the causative agent of Chagas' disease, preferentially infects cardiac and digestive tissues. Baboons living in Texas (Papio hamadryas) and cynomolgus monkeys (Macaca fascicularis) have been reported to be infected naturally with T. cruzi. In this study, we retrospectively reviewed cases of animals that were diagnosed with lymphocytic myocarditis and used a polymerase chain reaction (PCR)-based method (S36/S35 primer set) to amplify T. cruzi DNA from archived frozen and formalin-fixed paraffin-embedded (FFPE) cardiac tissues. We show that the PCR method is applicable in archived frozen and FFPE tissues and the sensitivity is in the femtogram range. A positive correlation between PCR positivity and lymphocytic myocarditis in both baboons and cynomolgus monkeys is shown. We also show epicarditis as a common finding in animals infected with T. cruzi.
- Subjects :
- Animals
Chagas Cardiomyopathy pathology
Chagas Cardiomyopathy veterinary
Female
Lymphocytes pathology
Macaca fascicularis
Male
Monkey Diseases parasitology
Myocarditis parasitology
Myocarditis pathology
Myocarditis veterinary
Papio hamadryas
Polymerase Chain Reaction
Retrospective Studies
Texas
Trypanosoma cruzi isolation & purification
Chagas Cardiomyopathy parasitology
DNA, Kinetoplast analysis
Trypanosoma cruzi genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1476-1645
- Volume :
- 90
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The American journal of tropical medicine and hygiene
- Publication Type :
- Academic Journal
- Accession number :
- 24567317
- Full Text :
- https://doi.org/10.4269/ajtmh.13-0448