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Cloning and expression of cDNA for human vascular anticoagulant, a Ca2+-dependent phospholipid-binding protein.
- Source :
-
European journal of biochemistry [Eur J Biochem] 1988 Jul 01; Vol. 174 (4), pp. 585-92. - Publication Year :
- 1988
-
Abstract
- Based on sequence information from tryptic peptides an almost full-size cDNA coding for the human vascular anticoagulant was isolated from a placental cDNA library and sequenced. The coding region was cloned into an Escherichia coli expression vector and the protein expressed at high levels. The recombinant protein was purified and found to be indistinguishable from its natural counterpart in several biological assays.
- Subjects :
- Amino Acid Sequence
Annexins
Base Sequence
Calcium metabolism
DNA analysis
Electrophoresis, Polyacrylamide Gel
Humans
Molecular Sequence Data
Peptides metabolism
Peptides pharmacology
Phospholipids metabolism
Placenta analysis
RNA analysis
Blood Coagulation drug effects
Cloning, Molecular
Peptides genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2956
- Volume :
- 174
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- European journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 2455636
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1988.tb14139.x