Combined analysis of the root bark oil of Cleistopholis glauca by chromatographic and spectroscopic techniques.

The composition of root bark oil from Cleistopholis glauca Pierre ex Engler & Diels growing wild in Ivory Coast was investigated by GC (in combination with retention indices) and "3C NMR spectroscopy after partition o f hydrocarbons a nd oxygenated compounds on silica g el. Thirty-one compounds havebeen identified. C. glauca produces a sesquiterpene-rich oil, patchoulenone (33.5%), cyperene (9.5%) and germacrene D (6.6%) being the main components. Special attention was paid to the identification and quantification ofgermacrene C (a heat-sensitive compound) and &-elemene, which were achieved by a combination of GC(FID) and 13C NMR spectroscopy. The composition of C. glauca root bark and leaf oils differed drastically.