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Osteopontin splice variant as a potential marker for metastatic disease in pancreatic adenocarcinoma.
- Source :
-
Journal of gastroenterology and hepatology [J Gastroenterol Hepatol] 2014 Jun; Vol. 29 (6), pp. 1321-7. - Publication Year :
- 2014
-
Abstract
- Background and Aim: Osteopontin (OPN) is a phosphoprotein that activates pathways that induce cancer cell survival and metastasis. Our aim was to examine the expression pattern of OPN splice variants a, b, and c in fine-needle aspirates and to determine their correlation with stage-adjusted pancreatic ductal adenocarcinoma (PDA) survival.<br />Methods: Endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) was performed in patients with solid pancreatic masses. The tissue was collected and analyzed for the expression of OPN isoforms by reverse transcription-polymerase chain reaction. Survival curves of stages and overexpression of OPN splice variants (a, b, c) were estimated according to the Kaplan-Meier and the log-rank test.<br />Results: EUS-FNA was performed in 46 patients with solid pancreatic lesions (40 PDA and 6 chronic pancreatitis). OPNa was highly expressed in 39/40 (98%), OPNb in 24/40 (60%), while OPNc was present in 10/40 (25%) of PDA samples. The median survival was lower in patients whose fine-needle aspiration (FNA) samples expressed OPNb than those without (406 days vs 749 days, P = 0.049). There was no significant difference in survival in patients with OPNc. Cox proportional hazard model demonstrated that OPNb expression had a trend toward decrease overall survival (P = 0.06), with these patients having a hazard of death three times higher than those without. OPNc was found to significantly correlate with metastatic disease (P = 0.009) in PDA patients.<br />Conclusions: Our data show for the first time that in FNA samples, there is a strong association between OPNc and presence of metastasis in PDA, and OPNb and poor survival.<br /> (© 2014 Journal of Gastroenterology and Hepatology Foundation and Wiley Publishing Asia Pty Ltd.)
- Subjects :
- Adenocarcinoma diagnosis
Adenocarcinoma mortality
Adult
Aged
Aged, 80 and over
Biomarkers, Tumor genetics
Biopsy, Fine-Needle
Endosonography
Female
Humans
Kaplan-Meier Estimate
Male
Middle Aged
Neoplasm Metastasis
Pancreatic Neoplasms diagnosis
Pancreatic Neoplasms mortality
Proportional Hazards Models
Protein Isoforms metabolism
Reverse Transcriptase Polymerase Chain Reaction
Survival Rate
Adenocarcinoma genetics
Adenocarcinoma pathology
Biomarkers, Tumor metabolism
Gene Expression
Genetic Association Studies
Genetic Variation
Osteopontin genetics
Osteopontin metabolism
Pancreatic Neoplasms genetics
Pancreatic Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1440-1746
- Volume :
- 29
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of gastroenterology and hepatology
- Publication Type :
- Academic Journal
- Accession number :
- 24548099
- Full Text :
- https://doi.org/10.1111/jgh.12561