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Caecal environment of rats fed far East Asian-modelled diets.
- Source :
-
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2014 May; Vol. 98 (10), pp. 4701-9. Date of Electronic Publication: 2014 Feb 16. - Publication Year :
- 2014
-
Abstract
- To clarify the effect of type of foods on the intestinal environment, Far East Asian- (FEA; rich in rice starch, soy protein and soy oil) and Far East Asian marine- (FEAM; rich in rice starch, fish meal, fish oil and brown alga) modelled diets and sucrose, casein and beef tallow-rich (SCB) diet were prepared. After the 2-week administration of diets in rats, caecal organic acids and putrefactive compounds (ammonia, indole, phenol and H2S, which are regarded as putative risk factors for tumours) were determined. The caecal microbiota was also analyzed using denaturing gradient gel electrophoresis and pyrosequencing with bar-coded primers targeting the bacterial 16S rRNA gene. Levels of n-butyrate, acetate, indole and phenol were high in rats fed FEA. On the other hand, H2S was clearly suppressed by both FEA and FEAM comparing with SCB. These results suggest that FEAM is preferable to FEA for optimal intestinal environment and host health. Both microbial analyses showed that the diversity of microbiota in the FEAM group was lower than in the other diet groups. Ratio of Firmicutes, Bacteroidetes and Proteobacteria in the SCB group was about 5:4:1. Firmicutes, particularly Lachnospiraceae, was promoted by FEA and FEAM.
- Subjects :
- Ammonia analysis
Animal Feed
Animals
Bacteria genetics
Cecum chemistry
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Denaturing Gradient Gel Electrophoresis
Hydrogen Sulfide
Molecular Sequence Data
Organic Chemicals analysis
RNA, Ribosomal, 16S genetics
Rats
Sequence Analysis, DNA
Bacteria classification
Biota
Cecum microbiology
Diet methods
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0614
- Volume :
- 98
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Applied microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 24531270
- Full Text :
- https://doi.org/10.1007/s00253-014-5535-8