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On-line two-step stacking in capillary zone electrophoresis for the preconcentration of strychnine and brucine.

Authors :
Yang X
Zhang S
Wang J
Wang C
Wang Z
Source :
Analytica chimica acta [Anal Chim Acta] 2014 Mar 03; Vol. 814, pp. 63-8. Date of Electronic Publication: 2014 Jan 21.
Publication Year :
2014

Abstract

An on-line sample preconcentration method by two-step stacking i.e., sweeping and micelle to solvent stacking, in capillary zone electrophoresis (CZE) has been developed for the determination of strychnine and brucine in traditional Chinese herbal medicines. After experimental optimizations, the best separation was achieved by using 75 mM phosphate buffer (pH 2.5) with 30% methanol (v/v). Compared with normal CZE injection, 51- and 38-fold improvement in concentration sensitivity was achieved for strychnine and brucine, respectively. The calibration curve was linear in the range of 0.1-5.0 μg mL(-1) for both strychnine and brucine, with the correlation coefficients of 0.9998 and 0.9997, respectively. The limits of detection (S/N=3) for both alkaloids were 0.01 μg mL(-1). The inter-day (n=8) and intra-day (n=5) reproducibilities expressed as the relative standard deviations for corrected peak area were less than 9.5%. The method was applied to determine strychnine and brucine in two Chinese herbal medicines, with recoveries ranging from 94.2% to 105.4%. The results indicated that the method is simple, rapid, reliable, and can be applied to determine strychnos alkaloids in traditional Chinese herbal medicines.<br /> (Copyright © 2014 Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1873-4324
Volume :
814
Database :
MEDLINE
Journal :
Analytica chimica acta
Publication Type :
Academic Journal
Accession number :
24528845
Full Text :
https://doi.org/10.1016/j.aca.2014.01.027