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Three nuclear genes suppress a yeast mitochondrial splice defect when present in high copy number.
- Source :
-
Current genetics [Curr Genet] 1987; Vol. 12 (7), pp. 503-9. - Publication Year :
- 1987
-
Abstract
- A gene bank of a yeast wild type DNA in the high copy number vector YEp13 was screened for recombinant plasmids which suppress the mitochondrial RNA splice defect exerted by mutant M1301, a -1 bp deletion in the first intron of the mitochondrial COB gene (bI1). A total of 17 recombinant plasmids with similar suppressor activity were found. Restriction mapping and cross-hybridization of the inserts revealed that these 17 plasmids contain three different inserts, all lacking any extended sequence homology. Each of the inserts, when present in high copy number, has a similar suppressor activity: high in the presence of mutation M1301 in bI1, a group II intron, and low but significant with the presence of few mutants in bI2 and bI3 of the COB gene, both of which are group I introns.
Details
- Language :
- English
- ISSN :
- 0172-8083
- Volume :
- 12
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Current genetics
- Publication Type :
- Academic Journal
- Accession number :
- 2452028
- Full Text :
- https://doi.org/10.1007/BF00419559