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Actively targeted in vivo multiplex detection of intrinsic cancer biomarkers using biocompatible SERS nanotags.
- Source :
-
Scientific reports [Sci Rep] 2014 Feb 12; Vol. 4, pp. 4075. Date of Electronic Publication: 2014 Feb 12. - Publication Year :
- 2014
-
Abstract
- Surface-enhanced Raman scattering (SERS) technique is becoming highly popular for multiplex biosensing due to the 'fingerprint' Raman spectra from every molecule. As a proof-of-concept, we demonstrated the actively targeted multiplex in vitro and in vivo detection of three intrinsic cancer biomarkers - EGFR, CD44 and TGFβRII in a breast cancer model using three multiplexing capable, biocompatible SERS nanoparticles/nanotags. Intra-tumorally injected antibody conjugated nanotags specifically targeting the three biomarkers exhibited maximum signal at 6 hours and no detectable signal at 72 hours. However, nanotags without antibodies showed no detectable signal after 6 hours. This difference could be due to the specific binding of the bioconjugated nanotags to the receptors on the cell surface. Thus, this study establishes SERS nanotags as an ultrasensitive nanoprobe for the multiplex detection of biomarkers and opens up its potential application in monitoring tumor progression and therapy and development into a theranostic probe.
- Subjects :
- Animals
Antibodies, Immobilized immunology
Biocompatible Materials chemistry
Biomarkers, Tumor immunology
Cell Line, Tumor
ErbB Receptors analysis
ErbB Receptors immunology
Female
Humans
Hyaluronan Receptors analysis
Hyaluronan Receptors immunology
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasms diagnosis
Protein Serine-Threonine Kinases analysis
Protein Serine-Threonine Kinases immunology
Receptor, Transforming Growth Factor-beta Type II
Receptors, Transforming Growth Factor beta analysis
Receptors, Transforming Growth Factor beta immunology
Transplantation, Heterologous
Biocompatible Materials metabolism
Biomarkers, Tumor analysis
Nanoparticles chemistry
Spectrum Analysis, Raman
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 4
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 24518045
- Full Text :
- https://doi.org/10.1038/srep04075