Back to Search Start Over

Nonthermal plasma affects viability and morphology of Mycoplasma hominis and Acholeplasma laidlawii.

Authors :
Ermolaeva SA
Rakovskaya IV
Miller GG
Sysolyatina EV
Mukhachev AY
Vasiliev MM
Adgamov RR
Levina GA
Petrov OF
Morfill GE
Grigoriev AI
Fortov VE
Gintsburg AL
Source :
Journal of applied microbiology [J Appl Microbiol] 2014 May; Vol. 116 (5), pp. 1129-36. Date of Electronic Publication: 2014 Feb 11.
Publication Year :
2014

Abstract

Aim: To study the effects exerted by argon microwave nonthermal plasma (NTP) on cell wall-lacking Mollicutes bacteria.<br />Methods and Results: 10(8) CFU ml(-1) agar plated Mycoplasma hominis and Acholeplasma laidlawii were treated with the nonthermal microwave argon plasma for 30-300 s. The maximal 10- and 100-fold drop was observed for A. laidlawii and Myc. hominis, respectively. Similarly treated Escherichia coli and Staphylococcus aureus demonstrated the 10(5) and 10(3) drop, respectively. Removal of cholesterol affected resistance of A. laidlawii. 10 mmol l(-1) antioxidant butylated hydroxytoluene decreased mortality by a factor of 25-200. UV radiation alone caused 25-85% mortality in comparison with the whole NTP. Exogenously added hydrogen peroxide H2O2 did not cause mortality. NTP treatment of Myc. hominis triggered growth of microcolonies, which were several tenfold smaller than a typical colony.<br />Conclusions: Despite the lack of cell wall, A. laidlawii and Myc. hominis were more resistant to argon microwave NTP than other tested bacteria. Mycoplasma hominis formed microcolonies upon NTP treatment. A role of UV and active species was demonstrated.<br />Significance and Impact of the Study: The first study of NTP effects on Mollicutes revealed importance of a membrane composition for bacterial resistance to NTP. New specific Myc. hominis morphological forms were observed. The study confirmed importance of the concerted action of reactive oxygen species (ROS) with UV and other plasma bioactive agents for NTP bactericidal action.<br /> (© 2014 The Society for Applied Microbiology.)

Details

Language :
English
ISSN :
1365-2672
Volume :
116
Issue :
5
Database :
MEDLINE
Journal :
Journal of applied microbiology
Publication Type :
Academic Journal
Accession number :
24517235
Full Text :
https://doi.org/10.1111/jam.12445