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The influence of exogenous antigenic stimulation on the specificity repertoire of background immunoglobulin-secreting cells of different isotypes.
- Source :
-
Cellular immunology [Cell Immunol] 1988 Apr 01; Vol. 112 (2), pp. 371-80. - Publication Year :
- 1988
-
Abstract
- The total number of spontaneously occurring ("background") IgM-, IgG-, and IgA-secreting cells and the frequency of antigen-specific IgM-, IgG-, and IgA-secreting cells were determined in germ-free BALB/c mice fed a chemically defined ultrafiltered diet (GF-CD), in specific pathogen-free BALB/c mice fed an autoclaved natural ingredient diet (SPF-NI), and in conventional BALB/c mice fed nonautoclaved natural ingredients (CV-NI). This was done by means of the ELISA-plaque assay. The results did not show differences among the various groups of mice with regard to the total numbers of IgM-secreting cells in the various lymphoid organs. Also the frequencies of IgM-secreting cells specific for DNP27-BSA and the anti-idiotypic monoclonal antibodies Ac38 and Ac146 did not differ significantly among GF-CD, SPF-NI, and CV-NI mice. GF-CD mice, however, did show substantially decreased numbers of IgG- and IgA-secreting cells in their lymphoid organs. Furthermore, there were striking differences in the frequencies of antigen-specific IgG- and IgA-secreting cells between GF-CD mice and the two other groups of mice. These results indicate that exogenous antigenic stimulation has a great effect on both the total numbers and the specificity repertoires of background IgG- and IgA-secreting cells. Such an influence could not be detected with regard to the background IgM-secreting cells. This suggests two distinct compartments of background Ig-secreting cells: a very stable, endogenously regulated compartment consisting mainly of IgM-secreting cells, and another compartment, consisting mainly of IgG- and IgA-secreting cells, whose numbers and specificity repertoire appeared to be influenced by exogenous antigenic stimulation.
- Subjects :
- Animals
Antibody-Producing Cells immunology
Antibody-Producing Cells metabolism
Dinitrophenols immunology
Enzyme-Linked Immunosorbent Assay
Female
Haptens immunology
Hemolytic Plaque Technique
Immunoglobulin Idiotypes immunology
Lymphoid Tissue cytology
Male
Mice
Mice, Inbred BALB C
Serum Albumin, Bovine immunology
Staphylococcal Protein A
Antibody-Producing Cells classification
Epitopes immunology
Immunoglobulin Isotypes biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0008-8749
- Volume :
- 112
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cellular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 2451571
- Full Text :
- https://doi.org/10.1016/0008-8749(88)90306-1