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Effects of diet-induced obesity on metabolic parameters and reproductive function in female Ossabaw minipigs.
- Source :
-
Comparative medicine [Comp Med] 2014 Feb; Vol. 64 (1), pp. 44-9. - Publication Year :
- 2014
-
Abstract
- This study characterizes the effect of an excess-calorie, high-fat, high-cholesterol, high-fructose diet on metabolic parameters and reproductive function in female Ossabaw minipigs. Cycling sows were fed a hypercaloric, high-fat, high-cholesterol, and high-fructose diet (obese, n = 4) or a control diet (control, n = 5) for 13 mo. During the final 4 mo, ovarian ultrasonography was done, blood was collected, and weights and measures were taken. Pigs then underwent ovarian stimulation. Cycle length and androstenedione, total testosterone, progesterone, estradiol, follicle-stimulating hormone, luteinizing hormone, insulin, fructosamine, lipid, and glucose levels were measured. In addition, adipose tissue aromatase gene expression was assessed. As compared with control pigs, obese pigs were hyperglycemic and hyperinsulinemic; had elevated total cholesterol, triglyceride, and leptin levels, and demonstrated abdominal adiposity. Visceral adipose tissue of obese pigs, as compared with control pigs, showed increased aromatase gene expression. Obese pigs had longer estrous cycles, higher serum androstenedione, and higher luteal phase serum luteinizing hormone, compared with control pigs. During the luteal phase, obese pigs had more medium, ovulatory, and cystic ovarian follicles, whereas control pigs had more small ovarian follicles. When fed an excess-calorie, high-fat, high-cholesterol, high-fructose diet, female Ossabaw minipigs develop obesity, metabolic syndrome, and abnormal reproductive function. This animal model may be applicable to studies of the effects of obesity on fertility in women.
- Subjects :
- Adipose Tissue enzymology
Adipose Tissue physiopathology
Adiposity
Animal Nutritional Physiological Phenomena
Animals
Aromatase metabolism
Biomarkers blood
Diet, High-Fat
Disease Models, Animal
Energy Intake
Estrous Cycle blood
Female
Fructose
Hormones blood
Infertility, Female blood
Infertility, Female pathology
Infertility, Female physiopathology
Maternal Nutritional Physiological Phenomena
Metabolic Syndrome blood
Obesity, Abdominal blood
Obesity, Abdominal physiopathology
Ovarian Follicle metabolism
Ovarian Follicle pathology
Ovulation Induction
Pregnancy
Time Factors
Cholesterol, Dietary
Energy Metabolism
Infertility, Female etiology
Metabolic Syndrome etiology
Obesity, Abdominal etiology
Reproduction
Swine blood
Swine, Miniature blood
Subjects
Details
- Language :
- English
- ISSN :
- 2769-819X
- Volume :
- 64
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Comparative medicine
- Publication Type :
- Academic Journal
- Accession number :
- 24512960