Blood group antigen staining pattern during experimental carcinogenesis in rat palate.

During 4-nitroquinoline-1-oxide-induced carcinogenesis in the rat palate, animals were sacrificed at various intervals and stained for blood group antigens B and H (Type 2 chain) by an immunofluorescent method. In rats without signs of epithelial dysplasia, the staining pattern was identical with that in the normal control rats. In rats with definite or questionable (borderline cases) dysplasias, marked changes in blood group antigen staining pattern were seen. Thus, changes in cell-surface carbohydrates during malignant development in the rat palate seem to follow closely the histomorphological changes. As there is good evidence that carcinomas would eventually develop in all rats if they were not sacrificed, it seems that the blood group antigen staining pattern does not predict malignant development in the absence of histological suspicion.