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Differentially expressed proteins in malignant and benign adrenocortical tumors.
- Source :
-
PloS one [PLoS One] 2014 Feb 03; Vol. 9 (2), pp. e87951. Date of Electronic Publication: 2014 Feb 03 (Print Publication: 2014). - Publication Year :
- 2014
-
Abstract
- We have compared the microsomal protein composition of eight malignant and six benign adrenocortical tumors with proteomic methods. IGF2 had increased level in the malignant tumors, confirming previous microarray studies on the same material. Aldolase A, a glycolytic enzyme, also showed increased levels in the malignant tissue compared to the benign. Additionally, several proteins belonging to complex I in the mitochondrial respiration chain showed decreased levels in the malignant tissue. Taken together, this may indicate a shift in energy metabolism where glycolysis may be favored over tight coupling of glycolysis and mitochondrial respiration, a phenomenon known as the Warburg effect. One of the complex I proteins that showed decreased levels in the malignant tissue was GRIM-19. This protein has been suggested as a tumor suppressive protein by being a negative regulator of STAT3. In summary, an analysis of the microsomal proteome in adrenocortical tumors identifies groups of proteins as well as specific proteins differentially expressed in the benign and malignant forms. These proteins shed light on the biology behind malignancy and could delineate future drug targets.
- Subjects :
- Blotting, Western
Chromatography, Liquid
Energy Metabolism
Humans
Immunoenzyme Techniques
Spectrometry, Mass, Electrospray Ionization
Adrenal Cortex Neoplasms metabolism
Biomarkers, Tumor metabolism
Insulin-Like Growth Factor II metabolism
Microsomes metabolism
Mitochondrial Proteins metabolism
Proteomics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 9
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 24498411
- Full Text :
- https://doi.org/10.1371/journal.pone.0087951