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Chemical fingerprinting of Codonopsis pilosula and simultaneous analysis of its major components by HPLC-UV.
- Source :
-
Archives of pharmacal research [Arch Pharm Res] 2014; Vol. 37 (9), pp. 1148-58. Date of Electronic Publication: 2014 Feb 05. - Publication Year :
- 2014
-
Abstract
- Although Codonopsis pilosula (C. pilosula) has long been considered as an important herbal medicine, no analytical method of marker compounds for quality assessment is registered in the Korean Pharmacopoeia. We developed a simple and robust analytical method of three marker components lobetyolin (1), lobetyol (2), and tangshenoside I (3) using HPLC-UV method. We also confirmed the three marker components using UPLC-qTOF/MS method. Various extraction conditions were optimized to achieve three marker compounds with faster extraction kinetics and higher recovery. The analytical condition was then validated by determining the linearity, accuracy, precision, limit of detection, limit of quantification, recovery, repeatability, robustness, and stability. By this method, the three markers were successfully quantified in 38 commercial samples along with three related species that are sometimes used as alternatives to C. pilosula. Finally, principal component and hierarchical clustering analyses were conducted to show the practicality of the method developed for the quality evaluation of C. pilosula.
- Subjects :
- Alkynes chemistry
Antihypertensive Agents isolation & purification
China
Chromatography, High Pressure Liquid
Cluster Analysis
Codonopsis growth & development
Disaccharides chemistry
Drugs, Chinese Herbal chemistry
Drugs, Chinese Herbal isolation & purification
Ethnopharmacology
Fatty Alcohols chemistry
Medicine, Korean Traditional
Molecular Structure
Plant Extracts isolation & purification
Polyynes chemistry
Principal Component Analysis
Quality Control
Reproducibility of Results
Republic of Korea
Species Specificity
Spectrometry, Mass, Electrospray Ionization
Spectrophotometry, Ultraviolet
Alkynes analysis
Antihypertensive Agents chemistry
Codonopsis chemistry
Disaccharides analysis
Fatty Alcohols analysis
Plant Extracts chemistry
Plant Roots chemistry
Polyynes analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1976-3786
- Volume :
- 37
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Archives of pharmacal research
- Publication Type :
- Academic Journal
- Accession number :
- 24497036
- Full Text :
- https://doi.org/10.1007/s12272-014-0335-3