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Hybrid molecules containing the A-domain of insulin-like growth factor-I and the B-chain of insulin have increased mitogenic activity relative to insulin.

Authors :
Tseng LY
Schwartz GP
Sheikh M
Chen ZZ
Joshi S
Wang JF
Nissley SP
Burke GT
Katsoyannis PG
Rechler MM
Source :
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1987 Dec 16; Vol. 149 (2), pp. 672-9.
Publication Year :
1987

Abstract

Two synthetic insulin-like compounds consisting of the B-chain of insulin linked via disulfide bonds to A chains corresponding to the A-domain or the A- and D-domains of insulin-like growth factor I (IGF-I) have been evaluated for mitogenic activity and for binding to IGF receptors and IGF carrier proteins. Both compounds are 3- to 5-fold more potent mitogens than insulin, and have a comparably increased affinity for the type I IGF receptor that mediates these mitogenic effects in chick embryo fibroblasts. Neither compound interacts with IGF carrier proteins. These results indicate that the A-domain of IGF-I is importantly involved in its growth-promoting properties.

Details

Language :
English
ISSN :
0006-291X
Volume :
149
Issue :
2
Database :
MEDLINE
Journal :
Biochemical and biophysical research communications
Publication Type :
Academic Journal
Accession number :
2447882
Full Text :
https://doi.org/10.1016/0006-291x(87)90420-7