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Circulating Mycobacterium bovis peptides and host response proteins as biomarkers for unambiguous detection of subclinical infection.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2014 Feb; Vol. 52 (2), pp. 536-43. Date of Electronic Publication: 2013 Dec 04. - Publication Year :
- 2014
-
Abstract
- Bovine tuberculosis remains one of the most damaging diseases to agriculture, and there is also a concern for human spillover. A critical need exists for rapid, thorough, and inexpensive diagnostic methods capable of detecting and differentiating Mycobacterium bovis infection from other pathogenic and environmental mycobacteria at multiple surveillance levels. In a previous study, Seth et al. (PLoS One 4:e5478, 2009, doi:10.1371/journal.pone.0005478) identified 32 host peptides that specifically increased in the blood serum of M. bovis-infected animals). In the current study, 16 M. bovis proteins were discovered in the blood serum proteomics data sets. A large-scale validation analysis was undertaken for selected host and M. bovis proteins using a cattle serum repository containing M. bovis (n = 128), Mycobacterium kansasii (n = 10), and Mycobacterium avium subsp. paratuberculosis (n = 10), cases exposed to M. bovis (n = 424), and negative controls (n = 38). Of the host biomarkers, vitamin D binding protein (VDBP) showed the greatest sensitivity and specificity for M. bovis detection. Circulating M. bovis proteins, specifically polyketide synthetase 5, detected M. bovis-infected cattle with little to no seroreactivity against M. kansasii- and M. avium subsp. paratuberculosis-infected animals. These data indicate that host and pathogen serum proteins can serve as reliable biomarkers for tracking M. bovis infection in animal populations.
- Subjects :
- Animals
Bacterial Proteins blood
Blood Chemical Analysis
Cattle
Latent Tuberculosis diagnosis
Proteome analysis
Sensitivity and Specificity
Vitamin D-Binding Protein blood
Biomarkers blood
Clinical Laboratory Techniques methods
Latent Tuberculosis veterinary
Mycobacterium bovis chemistry
Peptides blood
Tuberculosis, Bovine diagnosis
Veterinary Medicine methods
Subjects
Details
- Language :
- English
- ISSN :
- 1098-660X
- Volume :
- 52
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 24478485
- Full Text :
- https://doi.org/10.1128/JCM.02433-13