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B-1a cell diversity: nontemplated addition in B-1a cell Ig is determined by progenitor population and developmental location.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2014 Mar 01; Vol. 192 (5), pp. 2432-41. Date of Electronic Publication: 2014 Jan 29. - Publication Year :
- 2014
-
Abstract
- Natural Abs produced by B-1a cells are required for immediate protection against infection. The protective capacity of natural Abs is attributed to germline-like structure, which includes the relative absence of N-region addition. Previous studies have shown B-1a cell Ig from aged mice contains abundant nontemplated (N)-additions. B-1a cells have been shown to derive from a specific lineage-negative (Lin(-))CD45R(low/-)CD19(+) progenitor found both in fetal liver and adult bone marrow. In this study, we report identification of a fetal liver population characterized phenotypically as Lin(-)CD45R(-)CD19(-), which gives rise to IgM(+)IgD(low)CD45R(low)CD5(+)Mac-1(+)CD19(high)CD43(+)CD23(low) B-1a cells upon adoptive transfer to SCID recipients. These B-1a cells derived from the Lin(-)CD45R(-)CD19(-) fetal liver population produce natural Ab that binds pneumococcal Ags, but this Ig contains substantial N-addition despite initial absence of TdT. Furthermore, we show extensive N-addition is also present in B-1a cells derived from the Lin(-)CD45R(low/-)CD19(+) B-1 progenitor found in the bone marrow. Together these results demonstrate B-1a cell N-addition depends on the type of progenitor and the location of the progenitor during its development. These findings have implications for how regulation of different progenitors from fetal liver and bone marrow may play a role in the age-related increase in N-region addition by B-1a cells in normal animals.
- Subjects :
- Adoptive Transfer
Animals
Antigens, Bacterial immunology
Antigens, Differentiation immunology
B-Lymphocyte Subsets cytology
Fetus cytology
Fetus embryology
Liver cytology
Liver embryology
Male
Mice
Mice, Inbred BALB C
Mice, SCID
Streptococcus pneumoniae immunology
B-Lymphocyte Subsets immunology
Cell Lineage immunology
Fetus immunology
Liver immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 192
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 24477911
- Full Text :
- https://doi.org/10.4049/jimmunol.1300247