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Correlative super-resolution fluorescence and metal-replica transmission electron microscopy.
- Source :
-
Nature methods [Nat Methods] 2014 Mar; Vol. 11 (3), pp. 305-8. Date of Electronic Publication: 2014 Jan 26. - Publication Year :
- 2014
-
Abstract
- We combine super-resolution localization fluorescence microscopy with transmission electron microscopy of metal replicas to locate proteins on the landscape of the cellular plasma membrane at the nanoscale. We validate robust correlation on the scale of 20 nm by imaging endogenous clathrin (in two and three dimensions) and apply the method to find the previously unknown three-dimensional position of the endocytic protein epsin on clathrin-coated structures at the plasma membrane.
- Subjects :
- Adaptor Proteins, Vesicular Transport metabolism
Adaptor Proteins, Vesicular Transport ultrastructure
Clathrin ultrastructure
Humans
Membrane Proteins metabolism
Gold chemistry
Membrane Proteins ultrastructure
Microscopy, Electron, Transmission
Microscopy, Fluorescence
Nanotubes chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1548-7105
- Volume :
- 11
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Nature methods
- Publication Type :
- Academic Journal
- Accession number :
- 24464288
- Full Text :
- https://doi.org/10.1038/nmeth.2816