Dinuclear ruthenium(II) complexes as two-photon, time-resolved emission microscopy probes for cellular DNA.

Authors :: Baggaley E
Gill MR
Green NH
Turton D
Sazanovich IV
Botchway SW
Smythe C
Haycock JW
Weinstein JA
Thomas JA
Source :: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2014 Mar 24; Vol. 53 (13), pp. 3367-71. Date of Electronic Publication: 2014 Jan 23.
Publication Year :: 2014
Abstract: The first transition-metal complex-based two-photon absorbing luminescence lifetime probes for cellular DNA are presented. This allows cell imaging of DNA free from endogenous fluorophores and potentially facilitates deep tissue imaging. In this initial study, ruthenium(II) luminophores are used as phosphorescent lifetime imaging microscopy (PLIM) probes for nuclear DNA in both live and fixed cells. The DNA-bound probes display characteristic emission lifetimes of more than 160 ns, while shorter-lived cytoplasmic emission is also observed. These timescales are orders of magnitude longer than conventional FLIM, leading to previously unattainable levels of sensitivity, and autofluorescence-free imaging.<br /> (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Subjects :: DNA metabolism
Diagnostic Imaging
HeLa Cells
Humans
MCF-7 Cells
Microscopy methods
DNA chemistry
Ruthenium chemistry

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