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A new non-canonical pathway of Gα(q) protein regulating mitochondrial dynamics and bioenergetics.
- Source :
-
Cellular signalling [Cell Signal] 2014 May; Vol. 26 (5), pp. 1135-46. Date of Electronic Publication: 2014 Jan 18. - Publication Year :
- 2014
-
Abstract
- Contrary to previous assumptions, G proteins do not permanently reside on the plasma membrane, but are constantly monitoring the cytoplasmic surfaces of the plasma membrane and endomembranes. Here, we report that the Gαq and Gα11 proteins locate at the mitochondria and play a role in a complex signaling pathway that regulates mitochondrial dynamics. Our results provide evidence for the presence of the heteromeric G protein (Gαq/11βγ) at the outer mitochondrial membrane and for Gαq at the inner membrane. Both localizations are necessary to maintain the proper equilibrium between fusion and fission; which is achieved by altering the activity of mitofusin proteins, Drp1, OPA1 and the membrane potential at both the outer and inner mitochondrial membranes. As a result of the absence of Gαq/11, there is a decrease in mitochondrial fusion rates and a decrease in overall respiratory capacity, ATP production and OXPHOS-dependent growth. These findings demonstrate that the presence of Gαq proteins at the mitochondria serves as a physiological function: stabilizing elongated mitochondria and regulating energy production in Drp1 and Opa1 dependent mechanisms. This thereby links organelle dynamics and physiology.<br /> (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cell Line
Dynamins metabolism
GTP Phosphohydrolases metabolism
GTP-Binding Protein alpha Subunits, Gq-G11 antagonists & inhibitors
GTP-Binding Protein alpha Subunits, Gq-G11 genetics
HEK293 Cells
Humans
Membrane Potential, Mitochondrial
Mice
Mice, Transgenic
Mitochondria metabolism
Mitochondrial Dynamics
Mitochondrial Membranes metabolism
NIH 3T3 Cells
Oxidative Phosphorylation
Protein Subunits antagonists & inhibitors
Protein Subunits genetics
Protein Subunits metabolism
RNA, Small Interfering metabolism
Energy Metabolism
GTP-Binding Protein alpha Subunits, Gq-G11 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3913
- Volume :
- 26
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Cellular signalling
- Publication Type :
- Academic Journal
- Accession number :
- 24444709
- Full Text :
- https://doi.org/10.1016/j.cellsig.2014.01.009