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Ribosomal protein L13a deficiency in macrophages promotes atherosclerosis by limiting translation control-dependent retardation of inflammation.
- Source :
-
Arteriosclerosis, thrombosis, and vascular biology [Arterioscler Thromb Vasc Biol] 2014 Mar; Vol. 34 (3), pp. 533-42. Date of Electronic Publication: 2014 Jan 16. - Publication Year :
- 2014
-
Abstract
- Objective: Unresolved inflammatory response of macrophages plays a pivotal role in the pathogenesis of atherosclerosis. Previously we showed that ribosomal protein L13a-dependent translational silencing suppresses the synthesis of a cohort of inflammatory proteins in monocytes and macrophages. We also found that genetic abrogation of L13a expression in macrophages significantly compromised the resolution of inflammation in a mouse model of lipopolysaccharide-induced endotoxemia. However, its function in the pathogenesis of atherosclerosis is not known. Here, we examine whether L13a in macrophage has a protective role against high-fat diet-induced atherosclerosis.<br />Approach and Results: We bred the macrophage-specific L13a knockout mice L13a Flox(+/+) Cre(+/+) onto apolipoprotein E-deficient background and generated the experimental double knockout mice L13a Flox(+/+) Cre(+/+) apolipoprotein E deficient (apoE(-/-)). L13a Flox(+/+) Cre(-/-) mice on apolipoprotein E-deficient background were used as controls. Control and knockout mice were subjected to high-fat diet for 10 weeks. Evaluation of aortic sinus sections and entire aorta by en face showed significantly higher atherosclerosis in the knockout mice. Severity of atherosclerosis in knockout mice was accompanied by thinning of the smooth muscle cell layer in the media, larger macrophage area in the intimal plaque region and higher plasma levels of inflammatory cytokines. In addition, macrophages isolated from knockout mice had higher polyribosomal abundance of several target mRNAs, thus showing defect in translation control.<br />Conclusions: Our data demonstrate that loss of L13a in macrophages increases susceptibility to atherosclerosis in apolipoprotein E-deficient mice, revealing an important role of L13a-dependent translational control as an endogenous protection mechanism against atherosclerosis.
- Subjects :
- Animals
Aortic Diseases blood
Aortic Diseases genetics
Aortic Diseases pathology
Apolipoproteins E deficiency
Atherosclerosis blood
Atherosclerosis genetics
Atherosclerosis pathology
Chemotaxis, Leukocyte
Cholesterol blood
Cytokines blood
Dietary Fats toxicity
Disease Progression
Genetic Predisposition to Disease
Inflammation complications
Mice
Mice, Knockout
Peritoneum pathology
Polyribosomes metabolism
RNA, Messenger analysis
Ribosomal Proteins deficiency
Ribosomal Proteins genetics
Tunica Media pathology
Aortic Diseases etiology
Atherosclerosis etiology
Inflammation genetics
Macrophages metabolism
Protein Biosynthesis
Ribosomal Proteins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1524-4636
- Volume :
- 34
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Arteriosclerosis, thrombosis, and vascular biology
- Publication Type :
- Academic Journal
- Accession number :
- 24436370
- Full Text :
- https://doi.org/10.1161/ATVBAHA.113.302573