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Distribution of ESCRT machinery at HIV assembly sites reveals virus scaffolding of ESCRT subunits.
- Source :
-
Science (New York, N.Y.) [Science] 2014 Feb 07; Vol. 343 (6171), pp. 653-6. Date of Electronic Publication: 2014 Jan 16. - Publication Year :
- 2014
-
Abstract
- The human immunodeficiency virus (HIV) hijacks the endosomal sorting complexes required for transport (ESCRT) to mediate virus release from infected cells. The nanoscale organization of ESCRT machinery necessary for mediating viral abscission is unclear. Here, we applied three-dimensional superresolution microscopy and correlative electron microscopy to delineate the organization of ESCRT components at HIV assembly sites. We observed ESCRT subunits localized within the head of budding virions and released particles, with head-localized levels of CHMP2A decreasing relative to Tsg101 and CHMP4B upon virus abscission. Thus, the driving force for HIV release may derive from initial scaffolding of ESCRT subunits within the viral bud interior followed by plasma membrane association and selective remodeling of ESCRT subunits.
- Subjects :
- HIV-1 metabolism
Humans
Imaging, Three-Dimensional methods
Microscopy methods
Protein Subunits metabolism
Virion metabolism
Virus Release
gag Gene Products, Human Immunodeficiency Virus metabolism
Endosomal Sorting Complexes Required for Transport metabolism
HIV Infections virology
HIV-1 physiology
Virion physiology
Virus Assembly
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9203
- Volume :
- 343
- Issue :
- 6171
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 24436186
- Full Text :
- https://doi.org/10.1126/science.1247786