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Inaccuracy of AOAC method 2009.01 with amyloglucosidase for measuring non-digestible oligosaccharides and proposal for an improvement of the method.
- Source :
-
Food chemistry [Food Chem] 2014 May 15; Vol. 151, pp. 539-46. Date of Electronic Publication: 2013 Nov 27. - Publication Year :
- 2014
-
Abstract
- We wished to clarify the inaccuracy of AOAC method 2009.01 for the measurement of non-digestible oligosaccharides and to propose an improved method using porcine intestinal enzymes. Amyloglucosidase used in AOAC method 2009.01 scarcely hydrolyses sucrose, palatinose and panose (which are readily digested by intestinal enzymes). Hence, oligosaccharides could not be measured accurately by AOAC method 2009.01. To confirm the inaccuracy of the method, we used porcine intestinal enzymes instead of amyloglucosidase. Using the improved method, fructooligosaccharide and galactooligosaccharide were measured accurately as non-digestible oligosaccharides, but sucrose, palatinose, panose and isomaltooligosaccharide were not. The improved method hydrolysed digestible oligosaccharides into monosaccharides. These results demonstrate that the inaccuracy of AOAC method 2009.01 for oligosaccharide measurement is due to incomplete hydrolysis by amyloglucosidase. We propose that amyloglucosidase should be replaced with porcine intestinal enzymes for such measurements.<br /> (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Chromatography, High Pressure Liquid
Dietary Fiber analysis
Glucans analysis
Hydrolysis
Intestine, Small enzymology
Isomaltose analogs & derivatives
Isomaltose analysis
Sucrose analysis
Swine
Food Analysis methods
Glucan 1,4-alpha-Glucosidase analysis
Oligosaccharides analysis
Trisaccharides analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-7072
- Volume :
- 151
- Database :
- MEDLINE
- Journal :
- Food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 24423568
- Full Text :
- https://doi.org/10.1016/j.foodchem.2013.11.121