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Effect of power ultrasound pretreatment on peptidic profiles and angiotensin converting enzyme inhibition of milk protein concentrate hydrolysates.
- Source :
-
Journal of the science of food and agriculture [J Sci Food Agric] 2014 Sep; Vol. 94 (12), pp. 2420-8. Date of Electronic Publication: 2014 Mar 11. - Publication Year :
- 2014
-
Abstract
- Background: The use of power ultrasound as a pretreatment to enhance the hydrolysis of milk protein concentrate (MPC) and subsequent angiotensin converting enzyme (ACE) inhibitory activity has been studied. Liquid chromatography was used to analyse peptide profiles of Neutrase-derived MPC hydrolysates after pretreatment at 0, 1, 3, 5 and 8 min at an ultrasound power level of 800 W.<br />Results: The peptide profiles indicated an increase in number of peptides when ultrasound pretreatment was applied. There was also an increase in the degree of hydrolysis of MPC hydrolysates. The profiles indicated that new small peptides in ultrasound pretreated samples (1-5 min) which were not present in the control samples and 8 min pretreated samples, could be responsible for increased ACE inhibitory activity. These small peptides were digested in the 8 min pretreated samples.<br />Conclusion: Ultrasound pretreatment of MPC increases the ACE inhibitory activity of the hydrolysates because of the production of new small peptides. This can be used as a means to derive potent ACE inhibitory peptides at industrial scale in complex protein sources.<br /> (© 2014 Society of Chemical Industry.)
- Subjects :
- Animals
Hydrolysis
Metalloendopeptidases metabolism
Peptide Fragments metabolism
Peptide Hydrolases metabolism
Peptides pharmacology
Protein Hydrolysates pharmacology
Angiotensin-Converting Enzyme Inhibitors pharmacology
Milk chemistry
Milk Proteins metabolism
Peptides metabolism
Protein Hydrolysates metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1097-0010
- Volume :
- 94
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of the science of food and agriculture
- Publication Type :
- Academic Journal
- Accession number :
- 24415541
- Full Text :
- https://doi.org/10.1002/jsfa.6572