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Dimethyl sulfoxide attenuates hydrogen peroxide-induced injury in cardiomyocytes via heme oxygenase-1.
- Source :
-
Journal of cellular biochemistry [J Cell Biochem] 2014 Jun; Vol. 115 (6), pp. 1159-65. - Publication Year :
- 2014
-
Abstract
- The antioxidant property of dimethyl sulfoxide (DMSO) was formerly attributed to its direct effects. Our former study showed that DMSO is able to induce heme oxygenase-1 (HO-1) expression in endothelial cells, which is a potent antioxidant enzyme. In this study, we hypothesized that the antioxidant effects of DMSO in cardiomyocytes are mediated or partially mediated by increased HO-1 expression. Therefore, we investigated whether DMSO exerts protective effects against H2 O2 -induced oxidative damage in cardiomyocytes, and whether HO-1 is involved in DMSO-imparted protective effects, and we also explore the underlying mechanism of DMSO-induced HO-1 expression. Our study demonstrated that DMSO pretreatment showed a cytoprotective effect against H2 O2 -induced oxidative damage (impaired cell viability, increased apopototic cells rate and caspase-3 level, and increased release of LDH and CK) and this process is partially mediated by HO-1 upregulation. Furthermore, our data showed that the activation of p38 MAPK and Nrf2 translocation are involved in the HO-1 upregulation induced by DMSO. This study reports for the first time that the cytoprotective effect of DMSO in cardiomyocytes is partially mediated by HO-1, which may further explain the mechanisms by which DMSO exerts cardioprotection on H2 O2 injury. J. Cell. Biochem. 115: 1159-1165, 2014. © 2013 Wiley Periodicals, Inc.<br /> (© 2014 Wiley Periodicals, Inc.)
- Subjects :
- Active Transport, Cell Nucleus drug effects
Animals
Apoptosis drug effects
Blotting, Western
Caspase 3 metabolism
Cell Line
Cell Nucleus metabolism
Cell Survival drug effects
Cryoprotective Agents pharmacology
Myocytes, Cardiac cytology
Myocytes, Cardiac metabolism
NF-E2-Related Factor 2 metabolism
Oxidants pharmacology
Oxidative Stress drug effects
Rats
Up-Regulation drug effects
p38 Mitogen-Activated Protein Kinases metabolism
Dimethyl Sulfoxide pharmacology
Heme Oxygenase-1 metabolism
Hydrogen Peroxide pharmacology
Myocytes, Cardiac drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4644
- Volume :
- 115
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of cellular biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 24415199
- Full Text :
- https://doi.org/10.1002/jcb.24761