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Bio-hybrid silk fibroin/calcium phosphate/PLGA nanocomposite scaffold to control the delivery of vascular endothelial growth factor.
- Source :
-
Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2014 Feb 01; Vol. 35, pp. 401-10. Date of Electronic Publication: 2013 Dec 01. - Publication Year :
- 2014
-
Abstract
- This study investigated the efficacy of bio-hybrid silk fibroin/Calcium phosphate/PLGA nanocomposite scaffold as vascular endothelial growth factor (VEGF) delivery system. The scaffold was fabricated using freeze-drying and electrospinning. Here, we highlight the structural changes of the scaffold using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy and differential scanning calorimetry (DSC). The uniform dispersion of calcium phosohate (CaP) powder within silk fibroin (SF) solution was also confirmed using Zeta potential analysis. Moreover, good biocompatibility of osteoblast cells next to the scaffold was approved by cell adhesion, proliferation and alkaline phosphatase production. The release profile of VEGF during 28 days has established the efficacy of the scaffold as a sustained delivery system. The bioactivity of the released VEGF was maintained about 83%. The histology analysis has shown that the new bone tissue formation happened in the defected site after 10 weeks of implantation. Generally, our data showed that the fabricated scaffold could be considered as an effective scaffold for bone tissue engineering applications.<br /> (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Subjects :
- Biocompatible Materials chemical synthesis
Cell Proliferation drug effects
Cells, Cultured
Crystallization methods
Diffusion
Equipment Design
Equipment Failure Analysis
Humans
Lactic Acid chemistry
Materials Testing
Nanocapsules chemistry
Nanocomposites ultrastructure
Osteoblasts cytology
Osteoblasts drug effects
Polyglycolic Acid chemistry
Polylactic Acid-Polyglycolic Acid Copolymer
Surface Properties
Tissue Engineering instrumentation
Vascular Endothelial Growth Factor A chemistry
Calcium Phosphates chemistry
Fibroins chemistry
Nanocapsules administration & dosage
Nanocomposites chemistry
Osteoblasts physiology
Tissue Scaffolds
Vascular Endothelial Growth Factor A administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1873-0191
- Volume :
- 35
- Database :
- MEDLINE
- Journal :
- Materials science & engineering. C, Materials for biological applications
- Publication Type :
- Academic Journal
- Accession number :
- 24411394
- Full Text :
- https://doi.org/10.1016/j.msec.2013.11.023