Back to Search
Start Over
Protective activity of a novel resveratrol analogue, HS-1793, against DNA damage in 137Cs-irradiated CHO-K1 cells.
- Source :
-
Journal of radiation research [J Radiat Res] 2014 May; Vol. 55 (3), pp. 464-75. Date of Electronic Publication: 2014 Jan 07. - Publication Year :
- 2014
-
Abstract
- Resveratrol has received considerable attention as a polyphenol with anti-oxidant, anti-carcinogenic, and anti-inflammatory effects. Radiation is an important component of therapy for a wide range of malignant conditions. However, it causes damage to normal cells and, hence, can result in adverse side effects. This study was conducted to examine whether HS-1793, a novel resveratrol analogue free from the restriction of metabolic instability and the high dose requirement of resveratrol, induces a protective effect against radiation-induced DNA damage. HS-1793 effectively scavenged free radicals and inhibited radiation-induced plasmid DNA strand breaks in an in vitro assay. HS-1793 significantly decreased reactive oxygen species and cellular DNA damage in 2 Gy-irradiated Chinese hamster ovary (CHO)-K1 cells. In addition, HS-1793 dose-dependently reduced the levels of phosphorylated H2AX in irradiated CHO-K1 cells. These results indicate that HS-1793 has chemical radioprotective activity. Glutathione levels and superoxide dismutase activity in irradiated CHO-K1 cells increased significantly following HS-1793 treatment. The enhanced biological anti-oxidant activity and chemical radioprotective activity of HS-1793 maintained survival of irradiated CHO-K1 cells in a clonogenic assay. Therefore, HS-1793 may be of value as a radioprotector to protect healthy tissue surrounding tumor cells during radiotherapy to obtain better tumor control with a higher dose.
- Subjects :
- Animals
CHO Cells
Cell Survival drug effects
Cricetinae
Cricetulus
Dose-Response Relationship, Drug
Radiation Dosage
Radiation Tolerance drug effects
Radiation-Protective Agents administration & dosage
Cell Survival genetics
Cell Survival radiation effects
Cesium Radioisotopes pharmacology
DNA Damage drug effects
DNA Damage genetics
Naphthols administration & dosage
Radiation Tolerance physiology
Resorcinols administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1349-9157
- Volume :
- 55
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of radiation research
- Publication Type :
- Academic Journal
- Accession number :
- 24403520
- Full Text :
- https://doi.org/10.1093/jrr/rrt140