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Effects of hypotonic stress and ouabain on the apparent diffusion coefficient of water at cellular and tissue levels in Aplysia.
- Source :
-
NMR in biomedicine [NMR Biomed] 2014 Mar; Vol. 27 (3), pp. 280-90. Date of Electronic Publication: 2014 Jan 08. - Publication Year :
- 2014
-
Abstract
- There is evidence that physiological or pathological cell swelling is associated with a decrease of the apparent diffusion coefficient (ADC) of water in tissues, as measured with MRI. However the mechanism remains unclear. Magnetic resonance microscopy, performed on small tissue samples, has the potential to distinguish effects occurring at cellular and tissue levels. A three-dimensional diffusion prepared fast imaging with steady-state free precession sequence for MR microscopy was implemented on a 17.2 T imaging system and used to investigate the effect of two biological challenges known to cause cell swelling, exposure to a hypotonic solution or to ouabain, on Aplysia nervous tissue. The ADC was measured inside isolated neuronal soma and in the region of cell bodies of the buccal ganglia. Both challenges resulted in an ADC increase inside isolated neuronal soma (+31 ± 24% and +30 ± 11%, respectively) and an ADC decrease at tissue level in the buccal ganglia (-12 ± 5% and -18 ± 8%, respectively). A scenario involving a layer of water molecules bound to the inflating cell membrane surface is proposed to reconcile this apparent discrepancy.<br /> (Copyright © 2014 John Wiley & Sons, Ltd.)
- Subjects :
- Animals
Aplysia drug effects
Cell Nucleus drug effects
Cell Nucleus metabolism
Diffusion
Ganglia, Invertebrate cytology
Ganglia, Invertebrate drug effects
Ganglia, Invertebrate physiology
Holography
Magnetic Resonance Imaging
Phantoms, Imaging
Reproducibility of Results
Aplysia cytology
Aplysia physiology
Organ Specificity drug effects
Osmotic Pressure
Ouabain pharmacology
Water chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1099-1492
- Volume :
- 27
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- NMR in biomedicine
- Publication Type :
- Academic Journal
- Accession number :
- 24403001
- Full Text :
- https://doi.org/10.1002/nbm.3061