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Hypersensitive mAChRs are involved in the epiphora of transplanted glands.
- Source :
-
Journal of dental research [J Dent Res] 2014 Mar; Vol. 93 (3), pp. 306-12. Date of Electronic Publication: 2014 Jan 03. - Publication Year :
- 2014
-
Abstract
- Autologous transplantation of the submandibular gland is an effective treatment for severe dry eye syndrome. However, more than 40% of patients experience epiphora 3 to 6 months after transplantation. The underlying mechanism of epiphora remains to be elucidated. To investigate the potential roles of muscarinic acetylcholine receptors (mAChRs) in the induction of epiphora in transplanted glands, we assessed and found elevated mRNA and protein expression of M1- and M3-mAChR in transplanted glands from epiphora patients. The content of inositol 1, 4, 5-trisphosphate was also elevated. Moreover, carbachol (5 and 10 µM) induced greater increase of [Ca(2+)]i in isolated epiphora submandibular cells than in controls. Although aquaporin-5 (AQP5) content and distribution in the apical and lateral plasma of epiphora glands did not change, AQP5 content was reduced in lipid microdomains (lipid rafts and caveolae) but increased in non-lipid microdomains compared with controls. Carbachol (10 µM) increased the ratio of non-lipid microdomain to total AQP5 in the cultured control submandibular gland tissue. Taken together, these results indicated that hypersensitive mAChRs might be involved in the epiphora of transplanted submandibular glands by modulating AQP5 trafficking.
- Subjects :
- Adult
Aquaporin 5 analysis
Autografts drug effects
Calcium Signaling drug effects
Carbachol pharmacology
Caveolae drug effects
Caveolae pathology
Female
Humans
Inositol 1,4,5-Trisphosphate analysis
Male
Membrane Microdomains drug effects
Membrane Microdomains pathology
Middle Aged
Receptor, Muscarinic M1 analysis
Receptor, Muscarinic M3 analysis
Receptors, Muscarinic drug effects
Signal Transduction drug effects
Signal Transduction physiology
Submandibular Gland drug effects
Tissue Culture Techniques
Young Adult
Autografts transplantation
Dry Eye Syndromes surgery
Lacrimal Apparatus Diseases etiology
Postoperative Complications
Receptors, Muscarinic analysis
Submandibular Gland transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 1544-0591
- Volume :
- 93
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of dental research
- Publication Type :
- Academic Journal
- Accession number :
- 24389807
- Full Text :
- https://doi.org/10.1177/0022034513519107