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Combination use of ferulic acid, ligustrazine and tetrahydropalmatine inhibits the growth of ectopic endometrial tissue: a multi-target therapy for endometriosis rats.
- Source :
-
Journal of ethnopharmacology [J Ethnopharmacol] 2014 Feb 12; Vol. 151 (3), pp. 1218-1225. Date of Electronic Publication: 2014 Jan 02. - Publication Year :
- 2014
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Abstract
- Ethnopharmacological Relevance: Ferulic acid (FA), ligustrazine (LZ) and tetrahydropalmatine (THP) are separately isolated from Chinese Angelica, Szechwan Lovage Rhizome and Rhizoma in the Jiawei-Foshou-San formula, a popular traditional Chinese medicine for irregular menses. It has been reported that the combination use of FA+LZ+THP has similar effect on endometriosis, but the underlying mechanisms are unclear. This study was to investigate the combination effects and mechanisms of FA+LZ+THP on endometriosis rats.<br />Materials and Methods: Fifty endometriosis rats were intragastricly treated with FA+LZ+THP for 4 wk. The volume of ectopic endometrial tissue was measured to evaluate the effects. Then the changes in hypothalamic-pituitary-ovarian axis and ERE pathway were indicated by the levels of E2, GnRH, FSH and LH, and the expressions of ER, HSP90 and COX-2, respectively. In addition, peritoneal macrophages of each rat were cultured in vitro and treated with (FA+LZ+THP)-medicated serum for 24h. The proliferation and phagocytosis abilities, the levels of IL-1β and TNF-α, and the expression of IκBα were then measured for the changes of peritoneal macrophage activities.<br />Results: Combination use of FA+LZ+THP diminished the volume of the ectopic endometrial tissues (P<0.05 or P<0.01). It also decreased the E2 level, suppressed the expression of GnRH, FSH and LH, and decreased the protein expression of ER, HSP90 and COX-2 (all P<0.05 or P<0.01). The phagocytosis ability of peritoneal macrophage was enhanced by (FA+LZ+THP)-medicated serum (P<0.05) with no change of proliferation (P>0.05). Moreover, IL-1β and TNF-α were downregulated (both P<0.05 or P<0.01) and IκBα was upregulated by the (FA+LZ+THP)-medicated serum (P<0.01).<br />Conclusions: The combination use of FA, LZ and THP could inhibit the growth of ectopic endometrial tissue in endometriosis rats. It might be related to the down-regulation of hypothalamic-pituitary-ovarian axis, the amelioration in ERE pathway and the improvement of peritoneal macrophage activities.<br /> (Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Animals
Choristoma drug therapy
Choristoma metabolism
Drug Therapy, Combination
Endometriosis metabolism
Endometrium drug effects
Endometrium growth & development
Endometrium metabolism
Estradiol blood
Female
Follicle Stimulating Hormone metabolism
Gonadotropin-Releasing Hormone metabolism
I-kappa B Proteins metabolism
Interleukin-1beta metabolism
Luteinizing Hormone metabolism
Macrophages, Peritoneal drug effects
Macrophages, Peritoneal physiology
NF-KappaB Inhibitor alpha
Phagocytosis drug effects
Rats
Rats, Sprague-Dawley
Tumor Necrosis Factor-alpha metabolism
Berberine Alkaloids therapeutic use
Coumaric Acids therapeutic use
Endometriosis drug therapy
Pyrazines therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7573
- Volume :
- 151
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 24389027
- Full Text :
- https://doi.org/10.1016/j.jep.2013.12.047