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Atomic force microscope controlled topographical imaging and proximal probe thermal desorption/ionization mass spectrometry imaging.
- Source :
-
Analytical chemistry [Anal Chem] 2014 Jan 21; Vol. 86 (2), pp. 1083-90. Date of Electronic Publication: 2013 Dec 30. - Publication Year :
- 2014
-
Abstract
- This paper reports on the development of a hybrid atmospheric pressure atomic force microscopy/mass spectrometry imaging system utilizing nanothermal analysis probes for thermal desorption surface sampling with subsequent atmospheric pressure chemical ionization and mass analysis. The basic instrumental setup and the general operation of the system were discussed, and optimized performance metrics were presented. The ability to correlate topographic images of a surface with atomic force microscopy and a mass spectral chemical image of the same surface, utilizing the same probe without moving the sample from the system, was demonstrated. Co-registered mass spectral chemical images and atomic force microscopy topographical images were obtained from inked patterns on paper as well as from a living bacterial colony on an agar gel. Spatial resolution of the topography images based on pixel size (0.2 μm × 0.8 μm) was better than the resolution of the mass spectral images (2.5 μm × 2.0 μm), which were limited by current mass spectral data acquisition rate and system detection levels.
- Subjects :
- Agar
Atmospheric Pressure
Hot Temperature
Image Processing, Computer-Assisted
Ink
Microscopy, Atomic Force instrumentation
Phenazines analysis
Plant Roots microbiology
Populus microbiology
Printing
Pseudomonas chemistry
Pseudomonas metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization instrumentation
Surface Properties
Microscopy, Atomic Force methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6882
- Volume :
- 86
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 24377265
- Full Text :
- https://doi.org/10.1021/ac4026576