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Highly sensitive quantitative PCR for the detection and differentiation of Pseudogymnoascus destructans and other Pseudogymnoascus species.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 2014 Mar; Vol. 80 (5), pp. 1726-31. Date of Electronic Publication: 2013 Dec 27. - Publication Year :
- 2014
-
Abstract
- White-nose syndrome is a fungal disease that has decimated bat populations across eastern North America. Identification of the etiologic agent, Pseudogymnoascus destructans (formerly Geomyces destructans), in environmental samples is essential to proposed management plans. A major challenge is the presence of closely related species, which are ubiquitous in many soils and cave sediments and often present in high abundance. We present a dual-probe real-time quantitative PCR assay capable of detecting and differentiating P. destructans from closely related fungi in environmental samples from North America. The assay, based on a single nucleotide polymorphism (SNP) specific to P. destructans, is capable of rapid low-level detection from various sampling media, including sediment, fecal samples, wing biopsy specimens, and skin swabs. This method is a highly sensitive, high-throughput method for identifying P. destructans, other Pseudogymnoascus spp., and Geomyces spp. in the environment, providing a fundamental component of research and risk assessment for addressing this disease, as well as other ecological and mycological work on related fungi.
- Subjects :
- Ascomycota genetics
DNA, Fungal genetics
North America
Oligonucleotide Probes genetics
Polymorphism, Single Nucleotide
Sensitivity and Specificity
Ascomycota classification
Ascomycota isolation & purification
Environmental Microbiology
Mycology methods
Real-Time Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5336
- Volume :
- 80
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 24375140
- Full Text :
- https://doi.org/10.1128/AEM.02897-13