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Busulfan administration produces sublethal effects on somatic tissues and inhibits gametogenesis in Senegalese sole juveniles.
- Source :
-
Histology and histopathology [Histol Histopathol] 2014 May; Vol. 29 (5), pp. 619-28. Date of Electronic Publication: 2013 Dec 27. - Publication Year :
- 2014
-
Abstract
- Busulfan, a cytotoxic alkylating agent used for treatment of chronic myeloid leukemia has effects in mammalian germ cells. In fish species, the use of this compound is of special interest in intra and interspecies germ cell transplants. To determine the effects of busulfan in fish a previous range finding experiment was designed. Survival and growth rate of 150-days after hatching (150DAH) Senegalese sole (Solea senegalensis) juveniles was determined. In a second experiment, the effects of a sublethal busulfan dose in fish germ cell depletion and in somatic tissues were analysed. Sublethal effects of several busulfan treatments (B10-10 days after injection, B20-20 days after injection, B20÷-20 days after injection with double injection) were determined in somatic and gonadal tissues. Alterations were registered through histopathological techniques, TUNEL (cell apoptosis) and quantified at molecular level (Q-PCR analyses) using the vasa mRNAs (Ssvasa1-2 and Ssvasa3-4 mRNAs) as molecular markers for germinal cells in Senegalese sole juveniles. Several sublethal effects were observed with 40 mg kg⁻¹ busulfan, a non-lethal dose, such as: pyknosis in liver, increase of melanomacrophage centres and blood stagnation in spleen and interruption of gonadal development. Females were more affected by busulfan treatments than males in terms of germ cell disruption, since a significant decrease in the expression of both Ssvasa1-2 and Ssvasa3-4 markers was found in the gonad of treated females rather than males. At 10 days post-treatment (B10), females already presented a decrease in germ cell proliferation, as confirmed by Q-PCR. Ssvasa expression proved to be a reliable tool for the direct evaluation of the effects of busulfan on Senegalese sole gonadal development, proving that busulfan can be a suitable treatment for causing transient sterility in recipient gonads for germ cell transplantation.
- Subjects :
- Alkylating Agents toxicity
Animals
Breeding methods
Busulfan toxicity
Cell Proliferation drug effects
Dose-Response Relationship, Drug
Female
Flatfishes genetics
Gene Expression drug effects
Genetic Markers drug effects
Germ Cells drug effects
Germ Cells metabolism
Germ Cells transplantation
Gonads drug effects
Gonads metabolism
Gonads pathology
Male
RNA, Messenger genetics
RNA, Messenger metabolism
Reproductive Techniques, Assisted veterinary
Sterilization, Reproductive methods
Sterilization, Reproductive veterinary
Alkylating Agents administration & dosage
Busulfan administration & dosage
Flatfishes growth & development
Gametogenesis drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1699-5848
- Volume :
- 29
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Histology and histopathology
- Publication Type :
- Academic Journal
- Accession number :
- 24371034
- Full Text :
- https://doi.org/10.14670/HH-29.10.619