Back to Search
Start Over
Epizootiological survey of small mammals as Leptospira spp. reservoirs in Eastern Croatia.
- Source :
-
Acta tropica [Acta Trop] 2014 Mar; Vol. 131, pp. 111-6. Date of Electronic Publication: 2013 Dec 21. - Publication Year :
- 2014
-
Abstract
- In this survey we investigated a population of small mammals in Eastern Croatia in order to determine Leptospira carriage rates and identify circulating serovars. Out of 67 trapped animals, 20 (29.9%) isolates were obtained. Identification of isolates using microscopic agglutination test, pulsed field gel electrophoresis and multi locus sequence typing revealed that 10 (50.0%) isolates belong to serogroup Pomona, serovar Mozdok, 6 (30.0%) isolates to serogroup Australis, serovar Jalna, 2 (10.0%) isolates to serogroup Sejroe, serovar Saxkoebing, and 1 (5.0%) isolate to serogroup Grippotyphosa, serovar Grippotyphosa. One isolate from serogroup Bataviae was unable to be identified to the serovar level. Amplification of a 331-bp region of the locus LA0322 using real-time polymerase chain reaction determined that 12 (60.0%) isolates belong to L. kirschneri, 6 (30.0%) isolates to L. interrogans, and 2 (10.0%) isolates to L. borgpetersenii. Leptospira carriage rate was high (29.9%), which corresponds to a high incidence of human and domestic animal leptospirosis in Eastern Croatia. Furthermore, 90.0% of the isolates belong to serogroups Pomona, Australis and Sejroe which are also the most prevalent serogroups in humans in this area. These findings suggest that small mammals might be an important source of Leptospira spp. infection in Eastern Croatia.<br /> (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Subjects :
- Agglutination Tests
Animals
Croatia epidemiology
Electrophoresis, Gel, Pulsed-Field
Humans
Kidney microbiology
Leptospira genetics
Leptospirosis epidemiology
Leptospirosis microbiology
Multilocus Sequence Typing
Serotyping
Zoonoses
Leptospira classification
Leptospirosis veterinary
Phylogeny
Rodent Diseases
Rodentia microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-6254
- Volume :
- 131
- Database :
- MEDLINE
- Journal :
- Acta tropica
- Publication Type :
- Academic Journal
- Accession number :
- 24365042
- Full Text :
- https://doi.org/10.1016/j.actatropica.2013.12.009